Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation
Joint Authors
Wang, Peng
Chen, Fei
Wang, Wenyan
Zhang, Xue-Dong
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-24
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Hydrogen sulfide (H2S) has been shown to protect against oxidative stress injury and inflammation in various high glucose-induced insult models.
However, it remains unknown whether H2S protects human retinal pigment epithelial cells (RPE cells) from high glucose-induced damage.
In the current study, cell viability, proinflammatory cytokines, ROS, and inflammasome markers were compared in a low glucose- and high glucose-induced cell culture system.
The antioxidant N-acetylcysteine (NAC), NLRP3 siRNA, and NaHS were used to test RPE cell responses.
The results demonstrate that compared with the low-glucose culture, high glucose triggered higher cell death and increased IL-18 and IL-1β mRNA expression and protein production.
Furthermore, high glucose increased the mRNA expression levels of NLRP3, ACS, and caspase-1.
Notably, NAC, a ROS scavenger, could attenuate high glucose-induced ROS formation and IL-18 and IL-1β mRNA and protein expression and block inflammasome activation.
Silencing the NLRP3 gene expression also abolished IL-18 and IL-1β mRNA and protein expression.
Intrudingly, H2S could ameliorate high glucose-induced ROS formation, IL-18 and IL-1β expression, and inflammasome activation.
Taken together, the findings of the present study have demonstrated that H2S protects cultured RPE cells from high glucose-induced damage through inhibiting ROS formation and NLRP3 inflammasome activation.
It might suggest that H2S represents a potential therapeutic target for the treatment of diabetic retinopathy.
American Psychological Association (APA)
Wang, Peng& Chen, Fei& Wang, Wenyan& Zhang, Xue-Dong. 2019. Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation. Mediators of Inflammation،Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1193588
Modern Language Association (MLA)
Wang, Peng…[et al.]. Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation. Mediators of Inflammation No. 2019 (2019), pp.1-13.
https://search.emarefa.net/detail/BIM-1193588
American Medical Association (AMA)
Wang, Peng& Chen, Fei& Wang, Wenyan& Zhang, Xue-Dong. Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation. Mediators of Inflammation. 2019. Vol. 2019, no. 2019, pp.1-13.
https://search.emarefa.net/detail/BIM-1193588
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193588