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A Sequence-Dependent DNA Condensation Induced by Prion Protein
Joint Authors
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Different studies indicated that the prion protein induces hybridization of complementary DNA strands.
Cell culture studies showed that the scrapie isoform of prion protein remained bound with the chromosome.
In present work, we used an oxazole dye, YOYO, as a reporter to quantitative characterization of the DNA condensation by prion protein.
We observe that the prion protein induces greater fluorescence quenching of YOYO intercalated in DNA containing only GC bases compared to the DNA containing four bases whereas the effect of dye bound to DNA containing only AT bases is marginal.
DNA-condensing biological polyamines are less effective than prion protein in quenching of DNA-bound YOYO fluorescence.
The prion protein induces marginal quenching of fluorescence of the dye bound to oligonucleotides, which are resistant to condensation.
The ultrastructural studies with electron microscope also validate the biophysical data.
The GC bases of the target DNA are probably responsible for increased condensation in the presence of prion protein.
To our knowledge, this is the first report of a human cellular protein inducing a sequence-dependent DNA condensation.
The increased condensation of GC-rich DNA by prion protein may suggest a biological function of the prion protein and a role in its pathogenesis.
American Psychological Association (APA)
Bera, Alakesh& Biring, Sajal. 2018. A Sequence-Dependent DNA Condensation Induced by Prion Protein. Journal of Nucleic Acids،Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1193625
Modern Language Association (MLA)
Bera, Alakesh& Biring, Sajal. A Sequence-Dependent DNA Condensation Induced by Prion Protein. Journal of Nucleic Acids No. 2018 (2018), pp.1-14.
https://search.emarefa.net/detail/BIM-1193625
American Medical Association (AMA)
Bera, Alakesh& Biring, Sajal. A Sequence-Dependent DNA Condensation Induced by Prion Protein. Journal of Nucleic Acids. 2018. Vol. 2018, no. 2018, pp.1-14.
https://search.emarefa.net/detail/BIM-1193625
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193625