![](/images/graphics-bg.png)
Intramyocardial Injection of Recombinant Adeno-Associated Viral Vector Coexpressing PR39Adrenomedullin Enhances Angiogenesis and Reduces Apoptosis in a Rat Myocardial Infarction Model
Joint Authors
An, Rui
Xi, Cong
Xu, Jian
Liu, Ying
Zhang, Shumiao
Wang, Yuemin
Hao, Yuewen
Sun, Lijun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-02
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Cotransfer of angiogenic and antiapoptotic genes could be the basis of new gene therapy strategies for myocardial infarction.
In this study, rAAV-PR39-ADM, coexpressing antimicrobial peptide (PR39) and adrenomedullin (ADM), was designed with the mediation of recombinant adeno-associated virus.
In vitro, CRL-1730 cells were divided into four groups, namely, the sham group, the AAV-null group, the NS (normal saline) group, and the PR39-ADM group.
Immunocytochemistry analysis, CCK-8 assays, Matrigel assays, and apoptotic analysis were performed; in vivo, myocardial infarction model was established through ligation of the left coronary artery on rats, and treatment groups corresponded to those used in vitro.
Myocardial injury, cardiac performance, and the extent of myocardial apoptosis were assessed.
Results suggested that rAAV-PR39-ADM administration after myocardial infarction improved cell viability and cardiac function, attenuated apoptosis and myocardial injury, and promoted angiogenesis.
Subsequently, levels of 6×His, HIF-1α, VEGF, p-Akt, Akt, ADM, Bcl-2, and Bax were measured by western blot.
rAAV-PR39-ADM increased p-Akt, HIF-1α, and VEGF levels and induced higher Bcl-2 expression and lower Bax expression.
In conclusion, our results demonstrate that rAAV-PR39-ADM mitigates myocardial injury by promoting angiogenesis and reducing apoptosis.
This study suggests a potential novel gene therapy-based method that could be used clinically for myocardial infarction.
American Psychological Association (APA)
An, Rui& Xi, Cong& Xu, Jian& Liu, Ying& Zhang, Shumiao& Wang, Yuemin…[et al.]. 2017. Intramyocardial Injection of Recombinant Adeno-Associated Viral Vector Coexpressing PR39Adrenomedullin Enhances Angiogenesis and Reduces Apoptosis in a Rat Myocardial Infarction Model. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193759
Modern Language Association (MLA)
An, Rui…[et al.]. Intramyocardial Injection of Recombinant Adeno-Associated Viral Vector Coexpressing PR39Adrenomedullin Enhances Angiogenesis and Reduces Apoptosis in a Rat Myocardial Infarction Model. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1193759
American Medical Association (AMA)
An, Rui& Xi, Cong& Xu, Jian& Liu, Ying& Zhang, Shumiao& Wang, Yuemin…[et al.]. Intramyocardial Injection of Recombinant Adeno-Associated Viral Vector Coexpressing PR39Adrenomedullin Enhances Angiogenesis and Reduces Apoptosis in a Rat Myocardial Infarction Model. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193759
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193759