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Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently
Joint Authors
Koit, Andre
Shevchuk, Igor
Ounpuu, Lyudmila
Klepinin, Aleksandr
Chekulayev, Vladimir
Timohhina, Natalja
Tepp, Kersti
Puurand, Marju
Truu, Laura
Heck, Karoliina
Valvere, Vahur
Guzun, Rita
Kaambre, Tuuli
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-11
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
We conducted quantitative cellular respiration analysis on samples taken from human breast cancer (HBC) and human colorectal cancer (HCC) patients.
Respiratory capacity is not lost as a result of tumor formation and even though, functionally, complex I in HCC was found to be suppressed, it was not evident on the protein level.
Additionally, metabolic control analysis was used to quantify the role of components of mitochondrial interactosome.
The main rate-controlling steps in HBC are complex IV and adenine nucleotide transporter, but in HCC, complexes I and III.
Our kinetic measurements confirmed previous studies that respiratory chain complexes I and III in HBC and HCC can be assembled into supercomplexes with a possible partial addition from the complex IV pool.
Therefore, the kinetic method can be a useful addition in studying supercomplexes in cell lines or human samples.
In addition, when results from culture cells were compared to those from clinical samples, clear differences were present, but we also detected two different types of mitochondria within clinical HBC samples, possibly linked to two-compartment metabolism.
Taken together, our data show that mitochondrial respiration and regulation of mitochondrial membrane permeability have substantial differences between these two cancer types when compared to each other to their adjacent healthy tissue or to respective cell cultures.
American Psychological Association (APA)
Koit, Andre& Shevchuk, Igor& Ounpuu, Lyudmila& Klepinin, Aleksandr& Chekulayev, Vladimir& Timohhina, Natalja…[et al.]. 2017. Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1193795
Modern Language Association (MLA)
Koit, Andre…[et al.]. Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-16.
https://search.emarefa.net/detail/BIM-1193795
American Medical Association (AMA)
Koit, Andre& Shevchuk, Igor& Ounpuu, Lyudmila& Klepinin, Aleksandr& Chekulayev, Vladimir& Timohhina, Natalja…[et al.]. Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1193795
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193795