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Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos
Joint Authors
Leite, Roberta Ferreira
Annes, Kelly
Ispada, Jessica
de Lima, Camila Bruna
dos Santos, Érika Cristina
Fontes, Patricia Kubo
Nogueira, Marcelo Fábio Gouveia
Milazzotto, Marcella Pecora
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-25
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development.
This study evaluated the effect of different O2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development.
For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%).
Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus.
Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development.
Blastocyst rate was higher in the 5% O2 group and the number of cells was assessed, with the 5% O2 group having a higher number of cells.
ROS concentration was evaluated, with a higher ROS presence in the 20% O2 group.
Taken together, these results allow us to conclude that IVC of embryos at atmospheric O2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability.
American Psychological Association (APA)
Leite, Roberta Ferreira& Annes, Kelly& Ispada, Jessica& de Lima, Camila Bruna& dos Santos, Érika Cristina& Fontes, Patricia Kubo…[et al.]. 2017. Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1193832
Modern Language Association (MLA)
Leite, Roberta Ferreira…[et al.]. Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1193832
American Medical Association (AMA)
Leite, Roberta Ferreira& Annes, Kelly& Ispada, Jessica& de Lima, Camila Bruna& dos Santos, Érika Cristina& Fontes, Patricia Kubo…[et al.]. Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1193832
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193832