Mulberry Fruit Extract Affords Protection against Ethyl Carbamate-Induced Cytotoxicity and Oxidative Stress
Joint Authors
Chen, Wei
Li, Yuting
Bao, Tao
Gowd, Vemana
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-27
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Ethyl carbamate (EC) is a food and environmental toxicant and is a cause of concern for human exposure.
Several studies indicated that EC-induced toxicity was associated with oxidative stress.
Mulberry fruits are reported to have a wide range of bioactive compounds and pharmacological activities.
The present study was therefore aimed to investigate the protective property of mulberry fruit extract (MFE) on EC-induced cytotoxicity and oxidative stress.
Chemical composition analysis showed that total phenolic content and total flavonoid content in MFE were 502.43 ± 5.10 and 219.12 ± 4.45 mg QE/100 g FW.
Cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside were the major anthocyanins in MFE.
In vitro antioxidant studies (DPPH, ABTS, and FRAP assays) jointly exhibited the potent antioxidant capacity of MFE.
Further study indicated that MFE protected human liver HepG2 cells from EC-induced cytotoxicity by scavenging overproduced cellular ROS.
EC treatment promoted intracellular glutathione (GSH) depletion and caused mitochondrial membrane potential (MMP) collapse, as well as mitochondrial membrane lipid peroxidation, whereas MFE pretreatment significantly inhibited GSH depletion and restored the mitochondrial membrane function.
Overall, our study suggested that polyphenolic-rich MFE could afford a potent protection against EC-induced cytotoxicity and oxidative stress.
American Psychological Association (APA)
Chen, Wei& Li, Yuting& Bao, Tao& Gowd, Vemana. 2017. Mulberry Fruit Extract Affords Protection against Ethyl Carbamate-Induced Cytotoxicity and Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1193865
Modern Language Association (MLA)
Chen, Wei…[et al.]. Mulberry Fruit Extract Affords Protection against Ethyl Carbamate-Induced Cytotoxicity and Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1193865
American Medical Association (AMA)
Chen, Wei& Li, Yuting& Bao, Tao& Gowd, Vemana. Mulberry Fruit Extract Affords Protection against Ethyl Carbamate-Induced Cytotoxicity and Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1193865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193865