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Impact of Atherosclerosis- and Diabetes-Related Dicarbonyls on Vascular Endothelial Permeability: A Comparative Assessment
Joint Authors
Vorotnikov, A. V.
Samsonov, Mikhail V.
Khapchaev, Asker Y.
Vlasik, Tatyana N.
Yanushevskaya, Elena V.
Sidorova, Maria V.
Efremov, Evgeniy E.
Lankin, Vadim Z.
Shirinsky, Vladimir P.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Malondialdehyde (MDA), glyoxal (GO), and methylglyoxal (MGO) levels increase in atherosclerosis and diabetes patients.
Recent reports demonstrate that GO and MGO cause vascular endothelial barrier dysfunction whereas no evidence is available for MDA.
Methods.
To compare the effects of MDA, GO, or MGO on endothelial permeability, we used human EA.hy926 endothelial cells as a standard model.
To study cortical cytoplasm motility and cytoskeletal organization in endothelial cells, we utilized time-lapse microscopy and fluorescent microscopy.
To compare dicarbonyl-modified protein band profiles in these cells, we applied Western blotting with antibodies against MDA- or MGO-labelled proteins.
Results.
MDA (150–250 μM) irreversibly suppressed the endothelial cell barrier, reduced lamellipodial activity, and prevented intercellular contact formation.
The motile deficiency of MDA-challenged cells was accompanied by alterations in microtubule and microfilament organization.
These detrimental effects were not observed after GO or MGO (250 μM) administration regardless of confirmed modification of cellular proteins by MGO.
Conclusions.
Our comparative study demonstrates that MDA is more damaging to the endothelial barrier than GO or MGO.
Considering that MDA endogenous levels exceed those of GO or MGO and tend to increase further during lipoperoxidation, it appears important to reduce oxidative stress and, in particular, MDA levels in order to prevent sustained vascular hyperpermeability in atherosclerosis and diabetes patients.
American Psychological Association (APA)
Samsonov, Mikhail V.& Khapchaev, Asker Y.& Vorotnikov, A. V.& Vlasik, Tatyana N.& Yanushevskaya, Elena V.& Sidorova, Maria V.…[et al.]. 2017. Impact of Atherosclerosis- and Diabetes-Related Dicarbonyls on Vascular Endothelial Permeability: A Comparative Assessment. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1193881
Modern Language Association (MLA)
Samsonov, Mikhail V.…[et al.]. Impact of Atherosclerosis- and Diabetes-Related Dicarbonyls on Vascular Endothelial Permeability: A Comparative Assessment. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1193881
American Medical Association (AMA)
Samsonov, Mikhail V.& Khapchaev, Asker Y.& Vorotnikov, A. V.& Vlasik, Tatyana N.& Yanushevskaya, Elena V.& Sidorova, Maria V.…[et al.]. Impact of Atherosclerosis- and Diabetes-Related Dicarbonyls on Vascular Endothelial Permeability: A Comparative Assessment. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1193881
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193881