HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages
Joint Authors
Wang, Xin.
Yu, Weihua
Zhang, Xiaodi
Wu, Hao
Zhou, Qingbiao
Wang, Zhao
Liu, Rui
Liu, Jiangzheng
Hai, Chunxu
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-03
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG), an important monomer extracted from Polygonum multiflorum, can prevent a number of inflammation associated chronic diseases.
However, the mechanism involved in TSG inducing anti-inflammatory role remains unclear.
As an inducible antioxidant enzyme, Heme oxygenase-1 (HO-1), is crucial for protecting the mammalian cells against adverse stimuli.
Here, we found that the TSG treatment strongly induces the expression of HO-1 in an NRF2-depended manner.
Meanwhile, TSG increased the mitochondrial mass through upregulation of the mitochondrial biogenesis activators (PGC-1α, NRF1, and TFAM) as well as the mitochondrial complex IV.
Furthermore, TSG attenuated Lipopolysaccharide (LPS) mediated RAW264.7 cells activation and secretion of proinflammatory cytokines, including interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α).
Zinc Protoporphyrin (ZnPP), a selective inhibitor of HO-1 activity, was able to attenuate TSG mediated mitochondrial biogenesis and anti-inflammatory process.
Finally, we observed that LPS induced obvious mtDNA depletion and ATP deficiency, which indicated a severe damage of mitochondria.
TSG restored the LPS induced mitochondrial dysfunction via activation of the mitochondrial biogenesis.
ZnPP treatment markedly reversed the inhibitory effects of TSG on mitochondrial damage and oxidative stress in LPS stimulated macrophages.
Taken together, these findings suggest that TSG enhances mitochondrial biogenesis and function mainly via activation the HO-1.
TSG can be developed as a potential drug for treatment of inflammatory diseases.
American Psychological Association (APA)
Yu, Weihua& Zhang, Xiaodi& Wu, Hao& Zhou, Qingbiao& Wang, Zhao& Liu, Rui…[et al.]. 2017. HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193945
Modern Language Association (MLA)
Yu, Weihua…[et al.]. HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1193945
American Medical Association (AMA)
Yu, Weihua& Zhang, Xiaodi& Wu, Hao& Zhou, Qingbiao& Wang, Zhao& Liu, Rui…[et al.]. HO-1 Is Essential for Tetrahydroxystilbene Glucoside Mediated Mitochondrial Biogenesis and Anti-Inflammation Process in LPS-Treated RAW264.7 Macrophages. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1193945
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193945