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Oxidative Stress Delays PrometaphaseMetaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint
Joint Authors
Qian, Diting
Li, Zhiling
Wu, Que
Huang, Yue
Chen, Man
Xiao, Wanfen
Wang, Bin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In zygotes, DNA damage delays the first cleavage to enable repair.
Our previous study found that 0.03 mM hydrogen peroxide (H2O2) was the minimum concentration required for induction of oxidative DNA damage in mouse zygotes and that this represented the most similar situation to the clinical phenomenon.
In this study, we quantified the cleavage rates of cells in blastocysts at different developmental stages, followed by immunofluorescence to detect activation of γ-H2A histone family member X (a marker of DNA damage) in zygotes to confirm that oxidative DNA damage was induced in H2O2-treated zygotes.
Monitoring H3S10P (phosphorylation of Ser10 on histone H3; a prometaphase/metaphase marker) levels at different hour postinsemination revealed that treatment of zygotes with 0.03 mM H2O2 resulted in a prometaphase/metaphase delay.
Furthermore, immunofluorescence staining for mitotic arrest deficient 2-like 1 and the protein kinase TTK, components of the spindle assembly checkpoint (SAC), suggested that this delay possibly involved SAC activation.
These studies of the relationships between oxidative stress and SAC can promote the success rate of in vitro fertilization.
American Psychological Association (APA)
Wu, Que& Li, Zhiling& Huang, Yue& Qian, Diting& Chen, Man& Xiao, Wanfen…[et al.]. 2017. Oxidative Stress Delays PrometaphaseMetaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1193990
Modern Language Association (MLA)
Wu, Que…[et al.]. Oxidative Stress Delays PrometaphaseMetaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1193990
American Medical Association (AMA)
Wu, Que& Li, Zhiling& Huang, Yue& Qian, Diting& Chen, Man& Xiao, Wanfen…[et al.]. Oxidative Stress Delays PrometaphaseMetaphase of the First Cleavage in Mouse Zygotes via the MAD2L1-Mediated Spindle Assembly Checkpoint. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1193990
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1193990