Activation of Endocannabinoid Receptor 2 as a Mechanism of Propofol Pretreatment-Induced Cardioprotection against Ischemia-Reperfusion Injury in Rats
Joint Authors
Yao, Xue-Ya
Sun, Hai-Jing
Lu, Yan
Wang, Hao-Wei
Zhang, Hao
Wang, Shuang-Ran
Xu, Wen-Yun
Fu, Hai-Long
Yang, Feng
Yuan, Hong-Bin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-26
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Propofol pretreatment before reperfusion, or propofol conditioning, has been shown to be cardioprotective, while its mechanism is unclear.
The current study investigated the roles of endocannabinoid signaling in propofol cardioprotection in an in vivo model of myocardial ischemia/reperfusion (I/R) injury and in in vitro primary cardiomyocyte hypoxia/reoxygenation (H/R) injury.
The results showed that propofol conditioning increased both serum and cell culture media concentrations of endocannabinoids including anandamide (AEA) and 2-arachidonoylglycerol (2-AG) detected by LC-MS/MS.
The reductions of myocardial infarct size in vivo and cardiomyocyte apoptosis and death in vitro were accompanied with attenuations of oxidative injuries manifested as decreased reactive oxygen species (ROS), malonaldehyde (MDA), and MPO (myeloperoxidase) and increased superoxide dismutase (SOD) production.
These effects were mimicked by either URB597, a selective endocannabinoids degradation inhibitor, or VDM11, a selective endocannabinoids reuptake inhibitor.
In vivo study further validated that the cardioprotective and antioxidative effects of propofol were reversed by selective CB2 receptor antagonist AM630 but not CB1 receptor antagonist AM251.
We concluded that enhancing endogenous endocannabinoid release and subsequent activation of CB2 receptor signaling represent a major mechanism whereby propofol conditioning confers antioxidative and cardioprotective effects against myocardial I/R injury.
American Psychological Association (APA)
Sun, Hai-Jing& Lu, Yan& Wang, Hao-Wei& Zhang, Hao& Wang, Shuang-Ran& Xu, Wen-Yun…[et al.]. 2017. Activation of Endocannabinoid Receptor 2 as a Mechanism of Propofol Pretreatment-Induced Cardioprotection against Ischemia-Reperfusion Injury in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1194024
Modern Language Association (MLA)
Sun, Hai-Jing…[et al.]. Activation of Endocannabinoid Receptor 2 as a Mechanism of Propofol Pretreatment-Induced Cardioprotection against Ischemia-Reperfusion Injury in Rats. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-18.
https://search.emarefa.net/detail/BIM-1194024
American Medical Association (AMA)
Sun, Hai-Jing& Lu, Yan& Wang, Hao-Wei& Zhang, Hao& Wang, Shuang-Ran& Xu, Wen-Yun…[et al.]. Activation of Endocannabinoid Receptor 2 as a Mechanism of Propofol Pretreatment-Induced Cardioprotection against Ischemia-Reperfusion Injury in Rats. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-18.
https://search.emarefa.net/detail/BIM-1194024
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194024