Identification on Mantle Cell Lymphoma Using CD20 and CD5 Coupled Upconversion Fluorescent Nanoprobes
Joint Authors
Zhang, Mingkai
Gao, Yang
Wang, Jialiang
Liu, Zhanbo
Jin, Zaishun
Yu, Jianbo
Feng, Yukuan
Lü, Qiang
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-02
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In order to determine a particular tumor cell via nanomaterials, we introduce the preparation of CD20 and CD5 coupled nanoprobes (denoted as CD20 and CD5 nanoprobes for convenience) and an application in identification of mantle cell lymphoma (MCL) from B-cell lymphoma.
In this work, CD20 and CD5 nanoprobes were prepared by selectively oxidizing the carbon-carbon double bonds of oleate ligands on the surfaces of NaYF4:Yb3+,Tm3+ and NaYF4:Yb3+,Er3+ nanoparticles and, respectively, coupling carboxyl groups on the particles’ surfaces with CD20 and CD5 monoclonal antibodies through EDC/NHS crosslinking agents.
After in situ hybridized Jeko-1 cells and Raji cells as a reference with CD20 and CD5 nanoprobes, in vitro double-color upconversion fluorescence imaging of Jeko-1 cells was demonstrated through visualization of blue and green fluorescence under a 980 nm laser excitation.
Moreover, in vivo upconversion fluorescence imaging of the transplanted cancer model was also measured.
These experimental results indicate that Jeko-1 cells have been specifically labeled by CD20 and CD5 nanoprobes.
It is therefore concluded that CD20 and CD5 nanoprobes could be used to specially differentiate mantle cell lymphoma (MCL) from B-cell lymphoma.
American Psychological Association (APA)
Zhang, Mingkai& Gao, Yang& Wang, Jialiang& Liu, Zhanbo& Jin, Zaishun& Yu, Jianbo…[et al.]. 2018. Identification on Mantle Cell Lymphoma Using CD20 and CD5 Coupled Upconversion Fluorescent Nanoprobes. Journal of Nanomaterials،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1194080
Modern Language Association (MLA)
Zhang, Mingkai…[et al.]. Identification on Mantle Cell Lymphoma Using CD20 and CD5 Coupled Upconversion Fluorescent Nanoprobes. Journal of Nanomaterials No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1194080
American Medical Association (AMA)
Zhang, Mingkai& Gao, Yang& Wang, Jialiang& Liu, Zhanbo& Jin, Zaishun& Yu, Jianbo…[et al.]. Identification on Mantle Cell Lymphoma Using CD20 and CD5 Coupled Upconversion Fluorescent Nanoprobes. Journal of Nanomaterials. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1194080
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194080