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In Vivo Radioprotective Activity of Cell-Permeable Bifunctional Antioxidant Enzyme GST-TAT-SOD against Whole-Body Ionizing Irradiation in Mice
Joint Authors
Liu, Shu-Tao
Wu, Jun-xin
Pan, Jianru
He, Huocong
Su, Ying
Zheng, Guangjin
Rao, Pingfan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
GST-TAT-SOD was the fusion of superoxide dismutase (SOD), cell-permeable peptide TAT, and glutathione-S-transferase (GST).
It was proved to be a potential selective radioprotector in vitro in our previous work.
This study evaluated the in vivo radioprotective activity of GST-TAT-SOD against whole-body irradiation.
We demonstrated that intraperitoneal injection of 0.5 ml GST-TAT-SOD (2 kU/ml) 2 h before the 6 Gy whole-body irradiation in mice almost completely prevented the splenic damage.
It could significantly enhance the splenic antioxidant activity which kept the number of splenic white pulp and consequently resisted the shrinkage of the spleen.
Moreover, the thymus index, hepatic antioxidant activity, and white blood cell (WBC) count of peripheral blood in irradiated mice pretreated with GST-TAT-SOD also remarkably increased.
Although the treated and untreated irradiated mice showed no significant difference in the growth rate of animal body weight at 7 days postirradiation, the highest growth rate of body weight was observed in the GST-TAT-SOD-pretreated group.
Furthermore, GST-TAT-SOD pretreatment increased resistance against 8 Gy whole-body irradiation and enhanced 30 d survival.
The overall effect of GST-TAT-SOD seemed to be a bit more powerful than that of amifostine.
In conclusion, GST-TAT-SOD would be a safe and potentially promising radioprotector.
American Psychological Association (APA)
Pan, Jianru& He, Huocong& Su, Ying& Zheng, Guangjin& Wu, Jun-xin& Liu, Shu-Tao…[et al.]. 2017. In Vivo Radioprotective Activity of Cell-Permeable Bifunctional Antioxidant Enzyme GST-TAT-SOD against Whole-Body Ionizing Irradiation in Mice. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1194095
Modern Language Association (MLA)
Pan, Jianru…[et al.]. In Vivo Radioprotective Activity of Cell-Permeable Bifunctional Antioxidant Enzyme GST-TAT-SOD against Whole-Body Ionizing Irradiation in Mice. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1194095
American Medical Association (AMA)
Pan, Jianru& He, Huocong& Su, Ying& Zheng, Guangjin& Wu, Jun-xin& Liu, Shu-Tao…[et al.]. In Vivo Radioprotective Activity of Cell-Permeable Bifunctional Antioxidant Enzyme GST-TAT-SOD against Whole-Body Ionizing Irradiation in Mice. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1194095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194095