Validation of a Rapid Visual Screening of Campylobacter jejuni in Chicken Using Antibody-Conjugated Fluorescent Dye-Doped Silica Nanoparticle Reporters
Joint Authors
Poonlapdecha, Wanwisa
Seetang-Nun, Yortyot
Tuitemwong, Kooranee
Tuitemwong, Pravate
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The objective of this work was to validate the antibody-conjugated fluorescent dye-doped silica nanoparticle- (FDS-NP-) based assay for the rapid detection of Campylobacter jejuni in chicken samples using ISO16140:2003 single-laboratory validation guidelines.
This assay reversed the paradigm of microbial testing.
The noncultured FDS-NPs increased the fluorescent light signal of the test, not the cell numbers, and significantly reduced the detection time.
Validation results showed that relative detection level (LOD) of the assay was 103 cfu/ml.
The antibody-conjugated FDS-NPs were evaluated for the detection of C.
jejuni in 140 chicken samples collected from slaughterhouses (50 carcass rinse water, 60 rectal swabs, and 30 viscera contents) against ISO 10272 reference method with duplex PCR confirmation.
The relative accuracy (AC), relative specificity (SP), and relative sensitivity (SE) were 95.67%, 100%, and 94.87%, respectively.
The inclusivity test of C.
jejuni strains was 100% positive.
The exclusivity test demonstrated no cross-reactivity with 32 strains of non-Campylobacter strains.
The FDS-NP assay was very fast and specific, with time to result of 30 min compared with the 2–5 days required by the reference method.
The noncultured FDS-NPs demonstrated comparable performance to the cultured reference method to detect C.
jejuni in poultry samples.
It is applicable for effective screening of poultry product category.
American Psychological Association (APA)
Poonlapdecha, Wanwisa& Seetang-Nun, Yortyot& Tuitemwong, Kooranee& Tuitemwong, Pravate. 2018. Validation of a Rapid Visual Screening of Campylobacter jejuni in Chicken Using Antibody-Conjugated Fluorescent Dye-Doped Silica Nanoparticle Reporters. Journal of Nanomaterials،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1194138
Modern Language Association (MLA)
Poonlapdecha, Wanwisa…[et al.]. Validation of a Rapid Visual Screening of Campylobacter jejuni in Chicken Using Antibody-Conjugated Fluorescent Dye-Doped Silica Nanoparticle Reporters. Journal of Nanomaterials No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1194138
American Medical Association (AMA)
Poonlapdecha, Wanwisa& Seetang-Nun, Yortyot& Tuitemwong, Kooranee& Tuitemwong, Pravate. Validation of a Rapid Visual Screening of Campylobacter jejuni in Chicken Using Antibody-Conjugated Fluorescent Dye-Doped Silica Nanoparticle Reporters. Journal of Nanomaterials. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1194138
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194138