MicroRNA-34a: A Key Regulator in the Hallmarks of Renal Cell Carcinoma
Joint Authors
Fawzy, Manal S.
Toraih, Eman A.
Ibrahiem, Afaf T.
Hussein, Mohammad H.
Al-Qahtani, Saeed Awad M.
Shaalan, Aly A. M.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-21, 21 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-20
Country of Publication
Egypt
No. of Pages
21
Main Subjects
Abstract EN
Renal cell carcinoma (RCC) incidence has increased over the past two decades.
Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment.
This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC.
Quantitative real-time PCR for miR-34a and its targets; MET oncogene; gene-regulating apoptosis (TP53INP2 and DFFA); cell proliferation (E2F3); and cell differentiation (SOX2 and TGFB3) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens.
Clinicopathological parameter correlation and in silico network analysis have also implicated.
We found RCC tissues displayed significantly higher miR-34a expression level than their corresponding noncancerous tissues, particularly in chromophobic subtype.
MET and E2F3 were significantly upregulated, while TP53INP2 and SOX2 were downregulated.
ROC analysis showed high diagnostic performance of miR-34a (AUC = 0.854), MET (AUC = 0.765), and E2F3 (AUC = 0.761).
The advanced pathological grade was associated with strong TGFB1, VEGFA, and Ki67 protein expression and absent Tp53 staining.
These findings indicate miR-34a along with its putative target genes could play a role in RCC tumorigenesis and progression.
American Psychological Association (APA)
Toraih, Eman A.& Ibrahiem, Afaf T.& Fawzy, Manal S.& Hussein, Mohammad H.& Al-Qahtani, Saeed Awad M.& Shaalan, Aly A. M.. 2017. MicroRNA-34a: A Key Regulator in the Hallmarks of Renal Cell Carcinoma. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-21.
https://search.emarefa.net/detail/BIM-1194284
Modern Language Association (MLA)
Toraih, Eman A.…[et al.]. MicroRNA-34a: A Key Regulator in the Hallmarks of Renal Cell Carcinoma. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-21.
https://search.emarefa.net/detail/BIM-1194284
American Medical Association (AMA)
Toraih, Eman A.& Ibrahiem, Afaf T.& Fawzy, Manal S.& Hussein, Mohammad H.& Al-Qahtani, Saeed Awad M.& Shaalan, Aly A. M.. MicroRNA-34a: A Key Regulator in the Hallmarks of Renal Cell Carcinoma. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-21.
https://search.emarefa.net/detail/BIM-1194284
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194284