Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210
Joint Authors
Yan, Youyou
Liu, Bin
Tian, Xin
Zhao, Lei
Liu, Ning
Li, Tianyi
Song, Xianjin
Li, Zhibo
Zhang, Jichang
Shi, Yongfeng
Liu, Jia
Xiao, Yanlong
Sun, Wei
Guan, Yinuo
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Oxidative stress induces endothelial cell apoptosis and promotes atherosclerosis development.
MicroRNA-210 (miR-210) is linked with apoptosis in different cell types.
This study aimed to investigate the role of miR-210 in human umbilical vein endothelial cells (HUVECs) under oxidative stress and to determine the underlying mechanism.
HUVECs were treated with different concentrations of hydrogen peroxide (H2O2), and cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ATP assay.
To evaluate the role of miR-210 in H2O2-mediated apoptosis, gain-and-loss-of-function approaches were used, and the effects on apoptosis and reactive oxygen species (ROS) level were assayed using flow cytometry.
Moreover, miR-210 expression was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and expression of the following apoptosis-related genes was assessed by qRT-PCR and Western blot at the RNA and protein level, respectively: caspase-8-associated protein 2 (CASP8AP2), caspase-8, and caspase-3.
The results showed that H2O2 induced apoptosis in HUVECs in a dose-dependent manner and increased miR-210 expression.
Overexpression of miR-210 inhibited apoptosis and reduced ROS level in HUVECs treated with H2O2.
Furthermore, miR-210 downregulated CASP8AP2 and related downstream caspases at protein level.
Thus, under oxidative stress, miR-210 has a prosurvival and antiapoptotic effect on HUVECs by reducing ROS generation and downregulating the CASP8AP2 pathway.
American Psychological Association (APA)
Li, Tianyi& Song, Xianjin& Zhang, Jichang& Zhao, Lei& Shi, Yongfeng& Li, Zhibo…[et al.]. 2017. Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1194376
Modern Language Association (MLA)
Li, Tianyi…[et al.]. Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1194376
American Medical Association (AMA)
Li, Tianyi& Song, Xianjin& Zhang, Jichang& Zhao, Lei& Shi, Yongfeng& Li, Zhibo…[et al.]. Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1194376
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194376