Prevention of Streptozotocin-Induced Diabetic Nephropathy by MG132: Possible Roles of Nrf2 and IκB
Joint Authors
Kong, Li-li
Cui, Wenpeng
Miao, Lining
Luo, Manyu
Tan, Yi
Wang, Yang-wei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-08
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Our previous study showed that proteasomal inhibitor MG132 can prevent diabetic nephropathy (DN) along with upregulation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2).
The present study was to investigate whether MG132 can prevent DN in wild-type and Nrf2-KO mice.
Type 1 diabetes was induced in wild-type and Nrf2-KO mice by multiple low doses of streptozotocin.
Two weeks after streptozotocin injection, both wild-type and Nrf2-KO mice were randomly divided into four groups: control, MG132, DM, and DM/MG132.
MG132 (10 μg/kg/day) or vehicle was administered intraperitoneally for 4 months.
Renal function, morphology, and biochemical changes were measured after 4-month treatment with MG132.
MG132 treatment suppressed proteasomal activity in the two genotypes.
In wild-type mice, MG132 attenuated diabetes-induced renal dysfunction, fibrosis, inflammation, and oxidative damage along with increased Nrf2 and IκB expression.
Deletion of Nrf2 gene resulted in a partial, but significant attenuation of MG132 renal protection in Nrf2-KO mice compared with wild-type mice.
MG132-increased IκB expression was not different between wild-type and Nrf2-KO mice.
This work indicates that MG132 inhibits diabetes-increased proteasomal activity, resulting in Nrf2 and IκB upregulation and renal protection, which could be used as a strategy to prevent diabetic nephropathy.
American Psychological Association (APA)
Kong, Li-li& Wang, Yang-wei& Luo, Manyu& Tan, Yi& Cui, Wenpeng& Miao, Lining. 2017. Prevention of Streptozotocin-Induced Diabetic Nephropathy by MG132: Possible Roles of Nrf2 and IκB. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1194398
Modern Language Association (MLA)
Kong, Li-li…[et al.]. Prevention of Streptozotocin-Induced Diabetic Nephropathy by MG132: Possible Roles of Nrf2 and IκB. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1194398
American Medical Association (AMA)
Kong, Li-li& Wang, Yang-wei& Luo, Manyu& Tan, Yi& Cui, Wenpeng& Miao, Lining. Prevention of Streptozotocin-Induced Diabetic Nephropathy by MG132: Possible Roles of Nrf2 and IκB. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1194398
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194398