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Increasing the Fungicidal Action of Amphotericin B by Inhibiting the Nitric Oxide-Dependent Tolerance Pathway
Joint Authors
Vriens, Kim
Kumar, Phalguni Tewari
Struyfs, Caroline
Cools, Tanne L.
Spincemaille, Pieter
Kokalj, Tadej
Sampaio-Marques, Belém
Lammertyn, Jeroen
Giannattasio, Sergio
Cammue, Bruno P. A.
Thevissen, Karin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-10
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Amphotericin B (AmB) induces oxidative and nitrosative stresses, characterized by production of reactive oxygen and nitrogen species, in fungi.
Yet, how these toxic species contribute to AmB-induced fungal cell death is unclear.
We investigated the role of superoxide and nitric oxide radicals in AmB’s fungicidal activity in Saccharomyces cerevisiae, using a digital microfluidic platform, which enabled monitoring individual cells at a spatiotemporal resolution, and plating assays.
The nitric oxide synthase inhibitor L-NAME was used to interfere with nitric oxide radical production.
L-NAME increased and accelerated AmB-induced accumulation of superoxide radicals, membrane permeabilization, and loss of proliferative capacity in S.
cerevisiae.
In contrast, the nitric oxide donor S-nitrosoglutathione inhibited AmB’s action.
Hence, superoxide radicals were important for AmB’s fungicidal action, whereas nitric oxide radicals mediated tolerance towards AmB.
Finally, also the human pathogens Candida albicans and Candida glabrata were more susceptible to AmB in the presence of L-NAME, pointing to the potential of AmB-L-NAME combination therapy to treat fungal infections.
American Psychological Association (APA)
Vriens, Kim& Kumar, Phalguni Tewari& Struyfs, Caroline& Cools, Tanne L.& Spincemaille, Pieter& Kokalj, Tadej…[et al.]. 2017. Increasing the Fungicidal Action of Amphotericin B by Inhibiting the Nitric Oxide-Dependent Tolerance Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194531
Modern Language Association (MLA)
Vriens, Kim…[et al.]. Increasing the Fungicidal Action of Amphotericin B by Inhibiting the Nitric Oxide-Dependent Tolerance Pathway. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-17.
https://search.emarefa.net/detail/BIM-1194531
American Medical Association (AMA)
Vriens, Kim& Kumar, Phalguni Tewari& Struyfs, Caroline& Cools, Tanne L.& Spincemaille, Pieter& Kokalj, Tadej…[et al.]. Increasing the Fungicidal Action of Amphotericin B by Inhibiting the Nitric Oxide-Dependent Tolerance Pathway. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194531
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194531