Flavonoids as Putative Inducers of the Transcription Factors Nrf2, FoxO, and PPARγ
Joint Authors
Pallauf, Kathrin
Duckstein, Nils
Hasler, Mario
Klotz, Lars-Oliver
Rimbach, Gerald
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Dietary flavonoids have been shown to extend the lifespan of some model organisms and may delay the onset of chronic ageing-related diseases.
Mechanistically, the effects could be explained by the compounds scavenging free radicals or modulating signalling pathways.
Transcription factors Nrf2, FoxO, and PPARγ possibly affect ageing by regulating stress response, adipogenesis, and insulin sensitivity.
Using Hek-293 cells transfected with luciferase reporter constructs, we tested the potency of flavonoids from different subclasses (flavonols, flavones, flavanols, and isoflavones) to activate these transcription factors.
Under cell-free conditions (ABTS and FRAP assays), we tested their free radical scavenging activities and used α-tocopherol and ascorbic acid as positive controls.
Most of the tested flavonoids, but not the antioxidant vitamins, stimulated Nrf2-, FoxO-, and PPARγ-dependent promoter activities.
Flavonoids activating Nrf2 also tended to induce a FoxO and PPARγ response.
Interestingly, activation patterns of cellular stress response by flavonoids were not mirrored by their activities in ABTS and FRAP assays, which depended mostly on hydroxylation in the flavonoid B ring and, in some cases, extended that of the vitamins.
In conclusion, the free radical scavenging properties of flavonoids do not predict whether these molecules can stimulate a cellular response linked to activation of longevity-associated transcription factors.
American Psychological Association (APA)
Pallauf, Kathrin& Duckstein, Nils& Hasler, Mario& Klotz, Lars-Oliver& Rimbach, Gerald. 2017. Flavonoids as Putative Inducers of the Transcription Factors Nrf2, FoxO, and PPARγ. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1194719
Modern Language Association (MLA)
Pallauf, Kathrin…[et al.]. Flavonoids as Putative Inducers of the Transcription Factors Nrf2, FoxO, and PPARγ. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1194719
American Medical Association (AMA)
Pallauf, Kathrin& Duckstein, Nils& Hasler, Mario& Klotz, Lars-Oliver& Rimbach, Gerald. Flavonoids as Putative Inducers of the Transcription Factors Nrf2, FoxO, and PPARγ. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1194719
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194719