![](/images/graphics-bg.png)
miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1
Joint Authors
Wei, Zheng
Fang, Yi
Xie, Ting
Xue, Ning
Kuang, Qing
Liang, Mingyu
Ding, Xiaoqiang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-07
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Redox imbalance plays an important role in the pathogenesis of CKD progression.
Previously, we demonstrated that microRNA-382 (miR-382) contributed to TGF-β1-induced loss of epithelial polarity in human kidney epithelial cells, but its role in the development of renal tubulointerstitial fibrosis remains unknown.
In this study, we found that with 7 days of unilateral ureteral obstruction (UUO) in mice, the abundance of miR-382 in the obstructed kidney was significantly increased.
Meanwhile, the protein expression of heat shock protein 60 (HSPD1), a predicted target of miR-382, was reduced after 7 days of UUO.
Expression of 3-nitrotyrosine (3-NT) was upregulated, but expression of thioredoxin (Trx) was downregulated.
Anti-miR-382 treatment suppressed the upregulation of miR-382, attenuated renal interstitial fibrosis in the obstructed kidney, and reversed the downregulation of HSPD1/Trx and upregulation of 3-NT after UUO.
Furthermore, in vitro study revealed that overexpression of HSPD1 significantly restored Trx expression and reversed TGF-β1-induced loss of E-cadherin, while in vivo study found that direct siRNA-mediated suppression of HSPD1 in the UUO kidney promoted oxidative stress despite miR-382 blockade.
Our clinical data showed that upregulation of miR-382/3-NT and downregulation of HSPD1/Trx were also observed in IgA nephropathy patients with renal interstitial fibrosis.
These data supported a novel mechanism in which miR-382 targets HSPD1 and contributes to the redox imbalance in the development of renal fibrosis.
American Psychological Association (APA)
Fang, Yi& Xie, Ting& Xue, Ning& Kuang, Qing& Wei, Zheng& Liang, Mingyu…[et al.]. 2017. miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1194783
Modern Language Association (MLA)
Fang, Yi…[et al.]. miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-16.
https://search.emarefa.net/detail/BIM-1194783
American Medical Association (AMA)
Fang, Yi& Xie, Ting& Xue, Ning& Kuang, Qing& Wei, Zheng& Liang, Mingyu…[et al.]. miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1194783
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194783