Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
Joint Authors
Sainz de la Maza, D.
Moratilla, A.
Aparicio, V.
Lorca, C.
Alcaina, Y.
Martín, D.
De Miguel, M. P.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-05
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Cellular reprogramming is accompanied by a metabolic shift from oxidative phosphorylation (OXPHOS) toward glycolysis.
Previous results from our laboratory showed that hypoxia alone is able to reprogram primordial germ cells (PGCs) into pluripotency and that this action is mediated by hypoxia-inducible factor 1 (HIF1).
As HIF1 exerts a myriad of actions by upregulating several hundred genes, to ascertain whether the metabolic switch toward glycolysis is solely responsible for reprogramming, PGCs were cultured in the presence of a pyruvate kinase M2 isoform (PKM2) activator, or glycolysis was promoted by manipulating PPARγ.
Conversely, OXPHOS was stimulated by inhibiting PDK1 activity in normoxic or in hypoxic conditions.
Inhibition or promotion of autophagy and reactive oxygen species (ROS) production was performed to ascertain their role in cell reprogramming.
Our results show that a metabolic shift toward glycolysis, autophagy, and mitochondrial inactivation and an early rise in ROS levels are necessary for PGC reprogramming.
All of these processes are governed by HIF1/HIF2 balance and strict intermediate Oct4 levels.
Histone acetylation plays a role in reprogramming and is observed under all reprogramming conditions.
The pluripotent cells thus generated were unable to self-renew, probably due to insufficient Blimp1 downregulation and a lack of Klf4 and cMyc expression.
American Psychological Association (APA)
Sainz de la Maza, D.& Moratilla, A.& Aparicio, V.& Lorca, C.& Alcaina, Y.& Martín, D.…[et al.]. 2017. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194801
Modern Language Association (MLA)
Sainz de la Maza, D.…[et al.]. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-17.
https://search.emarefa.net/detail/BIM-1194801
American Medical Association (AMA)
Sainz de la Maza, D.& Moratilla, A.& Aparicio, V.& Lorca, C.& Alcaina, Y.& Martín, D.…[et al.]. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194801
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194801