Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating
Joint Authors
García Casillas, P. E.
Chapa Gonzalez, Christian
Zavala Tapia, Oscar
Villegas-Serralta, Enrique
Flores-Urquizo, Israel Alejandro
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Considerable effort has been focused on improving the control of size, shape, and surface modifications to detect proteins.
The purpose of this study was to compare the efficiencies of aminosilane-coated magnetite (As-M) nanoparticles (NPs), dextran-coated magnetite nanoparticles (Dx-M), and bare nanoparticles for conjugating single-chain variable fragment antibodies (scFvs) with the aim of detecting the human epidermal growth factor receptor 2 (HER2) protein.
Dx-M and As-M NPs were characterized using scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction, and Raman spectroscopy.
Dx-M and As-M were conjugated with a monoclonal scFv for active targeting of the HER2 antigen.
Aminosilane surface coating enhanced the scFv conjugation efficiency over twofold compared to that of the dextran-coated magnetite NPs for the detection of HER2 proteins.
American Psychological Association (APA)
Villegas-Serralta, Enrique& Zavala Tapia, Oscar& Flores-Urquizo, Israel Alejandro& García Casillas, P. E.& Chapa Gonzalez, Christian. 2018. Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating. Journal of Nanomaterials،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1194803
Modern Language Association (MLA)
Villegas-Serralta, Enrique…[et al.]. Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating. Journal of Nanomaterials No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1194803
American Medical Association (AMA)
Villegas-Serralta, Enrique& Zavala Tapia, Oscar& Flores-Urquizo, Israel Alejandro& García Casillas, P. E.& Chapa Gonzalez, Christian. Detection of HER2 through Antibody Immobilization Is Influenced by the Properties of the Magnetite Nanoparticle Coating. Journal of Nanomaterials. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1194803
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194803
