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Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E2 Receptor EP4 Pathway
Joint Authors
Yang, Chul Woo
Hong, Yu Ah
Park, Ki Cheol
Yang, Keum Jin
Jung, So Young
Choi, Hyunsu
Oh, Jeong Min
Lee, Sang Ju
Chang, Yoon Kyung
Kim, Suk Young
Hwang, Hyeon Seok
Park, Cheol Whee
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-29
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
The protective mechanism of paricalcitol remains unclear in renal ischemia-reperfusion (IR) injury.
We investigated the renoprotective effects of paricalcitol in IR injury through the prostaglandin E2 (PGE2) receptor EP4.
Paricalcitol was injected into IR-exposed HK-2 cells and mice subjected to bilateral kidney ischemia for 23 min and reperfusion for 24 hr.
Paricalcitol prevented IR-induced cell death and EP4 antagonist cotreatment offset these protective effects.
Paricalcitol increased phosphorylation of Akt and cyclic AMP responsive element binding protein (CREB) and suppressed nuclear factor-κB (NF-κB) in IR-exposed cells and cotreatment of EP4 antagonist or EP4 small interfering RNA blunted these signals.
In vivo studies showed that paricalcitol improved renal dysfunction and tubular necrosis after IR injury and cotreatment with EP4 antagonist inhibited the protective effects of paricalcitol.
Phosphorylation of Akt was increased and nuclear translocation of p65 NF-κB was decreased in paricalcitol-treated mice with IR injury, which was reversed by EP4 blockade.
Paricalcitol decreased oxidative stress and apoptosis in renal IR injury.
Paricalcitol also attenuated the infiltration of inflammatory cells and production of proinflammatory cytokines after IR injury.
EP4 antagonist abolished these antioxidant, anti-inflammatory, and antiapoptotic effects.
The EP4 plays a pivotal role in the protective effects of paricalcitol in renal IR injury.
American Psychological Association (APA)
Hong, Yu Ah& Yang, Keum Jin& Jung, So Young& Park, Ki Cheol& Choi, Hyunsu& Oh, Jeong Min…[et al.]. 2017. Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E2 Receptor EP4 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194834
Modern Language Association (MLA)
Hong, Yu Ah…[et al.]. Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E2 Receptor EP4 Pathway. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-17.
https://search.emarefa.net/detail/BIM-1194834
American Medical Association (AMA)
Hong, Yu Ah& Yang, Keum Jin& Jung, So Young& Park, Ki Cheol& Choi, Hyunsu& Oh, Jeong Min…[et al.]. Paricalcitol Pretreatment Attenuates Renal Ischemia-Reperfusion Injury via Prostaglandin E2 Receptor EP4 Pathway. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-17.
https://search.emarefa.net/detail/BIM-1194834
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194834