Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
Joint Authors
Lehmann, Sylvia G.
Bourgoin-Voillard, Sandrine
Seve, Michel
Rachidi, Walid
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-21, 21 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-23
Country of Publication
Egypt
No. of Pages
21
Main Subjects
Abstract EN
Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging.
Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level.
Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype.
We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women.
A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging.
Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways.
Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors.
Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging.
American Psychological Association (APA)
Lehmann, Sylvia G.& Bourgoin-Voillard, Sandrine& Seve, Michel& Rachidi, Walid. 2017. Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-21.
https://search.emarefa.net/detail/BIM-1194908
Modern Language Association (MLA)
Lehmann, Sylvia G.…[et al.]. Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-21.
https://search.emarefa.net/detail/BIM-1194908
American Medical Association (AMA)
Lehmann, Sylvia G.& Bourgoin-Voillard, Sandrine& Seve, Michel& Rachidi, Walid. Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-21.
https://search.emarefa.net/detail/BIM-1194908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194908