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Genetic Transformation and Hairy Root Induction Enhance the Antioxidant Potential of Lactuca serriola L.
Joint Authors
Ali, Hayssam M.
Elansary, Hosam O.
Elshikh, Mohamed S.
El-Esawi, Mohamed
Elkelish, Amr
Witczak, Jacques
Ahmad, Margaret
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lactuca serriola L.
is a herbaceous species, used for human nutrition and medicinal purposes.
The high antioxidant capacity of L.
serriola indicates the possibility of enhancing its edible and health potential by increasing the flavonoid and phenolic contents.
The present study aimed at enhancing the production of phenolics and flavonoids by hairy root cultures in Lactuca serriola transformed with Agrobacterium rhizogenes strain AR15834 harbouring the rolB gene.
The genetic transformation of rolB in transformed roots was validated, and rolB expression level was evaluated using real-time qPCR analysis.
Expression levels of flavonoid biosynthesis genes (CHI, PAL, FLS, and CHS) were assessed in the hairy and nontransformed roots.
Results showed higher expression levels in the transgenic roots than in the nontransformed ones (p<0.01).
Transgenic hairy roots exhibited a 54.8–96.7% increase in the total phenolic content, 38.1–76.2% increase in the total flavonoid content, and 56.7–96.7% increase in the total reducing power when compared with the nontransgenic roots (p<0.01).
DPPH results also revealed that the transgenic hairy roots exhibited a 31.6–50% increase in antioxidant potential, when compared to normal roots.
This study addressed the enhancement of secondary metabolite biosynthesis by hairy root induction in L.
serriola.
American Psychological Association (APA)
El-Esawi, Mohamed& Elkelish, Amr& Elansary, Hosam O.& Ali, Hayssam M.& Elshikh, Mohamed S.& Witczak, Jacques…[et al.]. 2017. Genetic Transformation and Hairy Root Induction Enhance the Antioxidant Potential of Lactuca serriola L.. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1194991
Modern Language Association (MLA)
El-Esawi, Mohamed…[et al.]. Genetic Transformation and Hairy Root Induction Enhance the Antioxidant Potential of Lactuca serriola L.. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1194991
American Medical Association (AMA)
El-Esawi, Mohamed& Elkelish, Amr& Elansary, Hosam O.& Ali, Hayssam M.& Elshikh, Mohamed S.& Witczak, Jacques…[et al.]. Genetic Transformation and Hairy Root Induction Enhance the Antioxidant Potential of Lactuca serriola L.. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1194991
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1194991