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Immediate Early Response Gene X-1 (IEX-1) Mediates Ischemic Preconditioning-Induced Cardioprotection in Rats
Joint Authors
Wang, Xian
Qu, Aijuan
Xu, Ming-Jiang
Cai, Yan
Shyy, John Y.-J.
Li, Wenjing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-29
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Reversible myocardial ischemia/reperfusion (I/R) or ischemic preconditioning (IPC) is associated with an immediate genomic response; IPC-induced immediate early genes are associated with reduced infarct size.
Because the immediate early response gene X-1 (IEX-1) plays a central role in cell apoptosis, we examine whether IEX-1 exerts protective effects against I/R injury.
We found that the IEX-1 mRNA level was increased in the IPC-imposed rat heart.
However, it was downregulated in the I/R rat heart, which was prevented by in situ IPC.
When IEX-1 was knocked down, the protective effects imposed by IPC were lessened.
Local gene delivery of Ad-IEX-1 to the left ventricle greatly diminished cardiac infarct size and improved systolic functions of I/R hearts in rats.
In contrast, knocking down IEX-1 expression exacerbates myocardial infarction.
Overexpression of IEX-1 in neonatal rat cardiomyocytes significantly reduced hypoxia-reoxygenation-induced intracellular and mitochondrial ROS accumulation and cell apoptosis.
Furthermore, IPC-induced phosphorylation and particle translocation of PKCε were impaired by knocking down IEX-1 in vivo, and overexpressing IEX-1 showed similar cardioprotection imposed by IPC.
Our results demonstrate that IPC increases IEX-1 expression, which may promote phosphorylation and particle translocation of PKCε and thus reduce intracellular ROS accumulation.
These beneficial effects reduce cardiomyocyte apoptosis and necrosis to alleviate cardiac infarction.
American Psychological Association (APA)
Xu, Ming-Jiang& Cai, Yan& Qu, Aijuan& Shyy, John Y.-J.& Li, Wenjing& Wang, Xian. 2017. Immediate Early Response Gene X-1 (IEX-1) Mediates Ischemic Preconditioning-Induced Cardioprotection in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1195172
Modern Language Association (MLA)
Xu, Ming-Jiang…[et al.]. Immediate Early Response Gene X-1 (IEX-1) Mediates Ischemic Preconditioning-Induced Cardioprotection in Rats. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1195172
American Medical Association (AMA)
Xu, Ming-Jiang& Cai, Yan& Qu, Aijuan& Shyy, John Y.-J.& Li, Wenjing& Wang, Xian. Immediate Early Response Gene X-1 (IEX-1) Mediates Ischemic Preconditioning-Induced Cardioprotection in Rats. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1195172
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195172