Low-Level Laser Irradiation Exerts Antiaggregative Effect on Human Platelets Independently on the Nitric Oxide Metabolism and Release of Platelet Activation Markers
Joint Authors
Mazur, Grzegorz
Skomro, Robert
Szahidewicz-Krupska, E.
Doroszko, A.
Derkacz, Arkadiusz
Dobrowolski, Piotr
Rola, Piotr
Rola, Paweł
Szymczyszyn, Alicja
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Aim.
The goal of the study is to develop a model allowing to investigate precisely the effect of low-level laser therapy (LLLT) on platelet aggregation and to verify the hypothesis regarding the role of the nitric oxide (NO) bioavailability and platelet activation markers in modulating platelet aggregation.
Methods.
A total of 41 healthy volunteers at the age of 21–45 years were investigated.
At first, platelet aggregation in response to three agonists (TRAP, ADP, and collagen) was evaluated following previous exposure to different doses of laser radiation (λ = 662 nm) to assess the dose-response effect.
Subsequently, plasma levels of platelet activation markers (PF4—platelet factor-4 and sP-selectin) as well as the substrate for nitric oxide synthase, L-arginine, and its competitive inhibitors (ADMA—asymmetric dimethylarginine and SDMA—symmetric dimethylarginine) were measured.
Results.
All doses of laser irradiation significantly reduced the aggregation.
However, the most pronounced effect was observed for 19.7 J/cm2.
No significant differences in the levels of platelet activation markers nor in the nitric-oxide-metabolic-pathway compounds between analyzed groups were noted.
Conclusions.
We have demonstrated in the established in vitro experimental model that the LLLT in a reproducible manner decreases the whole blood platelet aggregation regardless of the NO bioavailability or changes in the platelet activation markers.
American Psychological Association (APA)
Rola, Piotr& Doroszko, A.& Szahidewicz-Krupska, E.& Rola, Paweł& Dobrowolski, Piotr& Skomro, Robert…[et al.]. 2017. Low-Level Laser Irradiation Exerts Antiaggregative Effect on Human Platelets Independently on the Nitric Oxide Metabolism and Release of Platelet Activation Markers. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1195191
Modern Language Association (MLA)
Rola, Piotr…[et al.]. Low-Level Laser Irradiation Exerts Antiaggregative Effect on Human Platelets Independently on the Nitric Oxide Metabolism and Release of Platelet Activation Markers. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1195191
American Medical Association (AMA)
Rola, Piotr& Doroszko, A.& Szahidewicz-Krupska, E.& Rola, Paweł& Dobrowolski, Piotr& Skomro, Robert…[et al.]. Low-Level Laser Irradiation Exerts Antiaggregative Effect on Human Platelets Independently on the Nitric Oxide Metabolism and Release of Platelet Activation Markers. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1195191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195191