mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress
Joint Authors
Xia, Yiru
Sun, Mengjun
Xie, Yufeng
Shu, Rong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The aging periodontium may be vulnerable to periodontal pathogens and poor response to inflammation and susceptible to tumorigenesis.
Human gingival fibroblasts (hGFs) through continuously replicative culture served as an in vitro surrogate for aging.
To investigate the effects of the mechanistic target of rapamycin (mTOR) inhibition on the aging gingiva, we stimulated the high-passage hGFs with rapamycin (20 nmol/L) for 3 days and 30 days.
The cellular and biological changes were examined by immunofluorescence, real-time PCR, ELISA, Western blotting, and flow cytometry.
The data demonstrated that the inhibition of mTOR signaling led to fewer senescence-associated beta-galactosidase- (SA-β-Gal-) positive cells, delayed the onset of senescence, preserved the capability of proliferation, and lowered the expression levels of relevant senescence-associated markers, such as p16INK4a, p21CIP1a, interleukin-6 (IL-6), and IL-8.
In addition, when infected by prominent periodontal pathogens, Porphyromonas gingivalis (ATCC 33277), rapamycin-pretreated groups decreased the expression of inflammatory cytokines (IL-6 and IL-8) compared with the control group.
mTOR inhibition upregulated the gene expression of antioxidant components (Cat, Sod2, and Prdx3; P<0.05) and consequently neutralized the excessive reactive oxygen species (ROS).
In conclusion, our results indicated that mTOR inhibition might rejuvenate the aging gingiva to some extent and relieve inflammation through eliminating oxidative stress.
American Psychological Association (APA)
Xia, Yiru& Sun, Mengjun& Xie, Yufeng& Shu, Rong. 2017. mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195218
Modern Language Association (MLA)
Xia, Yiru…[et al.]. mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1195218
American Medical Association (AMA)
Xia, Yiru& Sun, Mengjun& Xie, Yufeng& Shu, Rong. mTOR Inhibition Rejuvenates the Aging Gingival Fibroblasts through Alleviating Oxidative Stress. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195218