Long-Term Treatment of Native LDL Induces Senescence of Cultured Human Endothelial Cells
Joint Authors
Oh, Sung-Tack
Park, Hoon
Yoon, Hyun Joong
Yang, Sung Yeul
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-19
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The study aimed to evaluate whether the treatment of primary cultured human endothelial cells with native low-density lipoprotein (nLDL) could induce their senescence and to uncover some of the putative mechanisms involved.
For this purpose, human umbilical vein endothelial cells (HUVECs) were subcultured and/or continuously cultured with nLDL (0, 2, 5, and 10 μg protein/mL), for up to 9 days.
The results indicated that nLDL inhibited the proliferation of HUVECs by arresting the cell cycle at G1 phase.
The G1-arrested cells showed increase in cytosolic senescence-associated-β-galactosidase (SA-β-Gal) activity, a biomarker of cellular senescence.
The causative factor of the cellular senescence was nLDL itself and not oxidized LDL (oxLDL), since blocking LDL receptor (LDLR) with the anti-LDLR antibody opposed the nLDL-induced increase of SA-β-Gal activity and decrease of cellular proliferation.
In addition, nLDL-induced cellular senescence by inhibiting the phosphorylation of pRb (G1 arrest) via p53 as well as p16 signal transduction pathways.
G1 phase arrest of the senescent cells was not overcome by nLDL removal from the culture medium.
Moreover, the nLDL-treated cells produced reactive oxygen species (ROS) dose- and time-dependently.
These results suggested, for the first time, that long-term treatment of nLDL could induce the premature senescence of endothelial cells.
American Psychological Association (APA)
Oh, Sung-Tack& Park, Hoon& Yoon, Hyun Joong& Yang, Sung Yeul. 2017. Long-Term Treatment of Native LDL Induces Senescence of Cultured Human Endothelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195253
Modern Language Association (MLA)
Oh, Sung-Tack…[et al.]. Long-Term Treatment of Native LDL Induces Senescence of Cultured Human Endothelial Cells. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1195253
American Medical Association (AMA)
Oh, Sung-Tack& Park, Hoon& Yoon, Hyun Joong& Yang, Sung Yeul. Long-Term Treatment of Native LDL Induces Senescence of Cultured Human Endothelial Cells. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1195253
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195253