Biosynthesis of the Essential Fatty Acid Oxidation Cofactor Carnitine Is Stimulated in Heart and Liver after a Single Bout of Exercise in Mice
Joint Authors
Broderick, Tom L.
Cusimano, Frank A.
Carlson, Chelsea
Babu, Jeganathan Ramesh
Source
Journal of Nutrition and Metabolism
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We determined whether one single bout of exercise stimulates carnitine biosynthesis and carnitine uptake in liver and heart.
Free carnitine (FC) in plasma was assayed using acetyltransferase and [14C]acetyl-CoA in Swiss Webster mice after 1 hour of moderate-intensity treadmill running or 4 hours and 8 hours into recovery.
Liver and heart were removed under the same conditions for measurement of carnitine biosynthesis enzymes (liver butyrobetaine hydroxylase, γ-BBH; heart trimethyllysine dioxygenase, TMLD), organic cation transporter-2 (OCTN2, carnitine transporter), and liver peroxisome proliferator-activated receptor-alpha (PPARα, transcription factor for γ-BBH and OCTN2 synthesis).
In exercised mice, FC levels in plasma decreased while heart and liver OCTN2 protein expressed increased, reflecting active uptake of FC.
During recovery, the rise in FC to control levels was associated with increased liver γ-BBH expression.
Protein expression of PPARα was stimulated in liver after exercise and during recovery.
Interestingly, heart TMLD protein was also detected after exercise.
Acute exercise stimulates carnitine uptake in liver and heart.
The rapid return of FC levels in plasma after exercise indicates carnitine biosynthesis by liver is stimulated to establish carnitine homeostasis.
Our results suggest that exercise may benefit patients with carnitine deficiency syndromes.
American Psychological Association (APA)
Broderick, Tom L.& Cusimano, Frank A.& Carlson, Chelsea& Babu, Jeganathan Ramesh. 2018. Biosynthesis of the Essential Fatty Acid Oxidation Cofactor Carnitine Is Stimulated in Heart and Liver after a Single Bout of Exercise in Mice. Journal of Nutrition and Metabolism،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1195368
Modern Language Association (MLA)
Broderick, Tom L.…[et al.]. Biosynthesis of the Essential Fatty Acid Oxidation Cofactor Carnitine Is Stimulated in Heart and Liver after a Single Bout of Exercise in Mice. Journal of Nutrition and Metabolism No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1195368
American Medical Association (AMA)
Broderick, Tom L.& Cusimano, Frank A.& Carlson, Chelsea& Babu, Jeganathan Ramesh. Biosynthesis of the Essential Fatty Acid Oxidation Cofactor Carnitine Is Stimulated in Heart and Liver after a Single Bout of Exercise in Mice. Journal of Nutrition and Metabolism. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1195368
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195368