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Estrogens Protect Calsequestrin-1 Knockout Mice from Lethal Hyperthermic Episodes by Reducing Oxidative Stress in Muscle
Joint Authors
Reggiani, Carlo
Canato, M.
Protasi, Feliciano
Boncompagni, Simona
Michelucci, Antonio
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-10
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Oxidative stress has been proposed to play a key role in malignant hyperthermia (MH), a syndrome caused by excessive Ca2+ release in skeletal muscle.
Incidence of mortality in male calsequestrin-1 knockout (CASQ1-null) mice during exposure to halothane and heat (a syndrome closely resembling human MH) is far greater than that in females.
To investigate the possible role of sex hormones in this still unexplained gender difference, we treated male and female CASQ1-null mice for 1 month, respectively, with Premarin (conjugated estrogens) and leuprolide (GnRH analog) and discovered that during exposure to halothane and heat Premarin reduced the mortality rate in males (79–27% and 86–20%), while leuprolide increased the incidence of mortality in females (18–73% and 24–82%).
We then evaluated the (a) responsiveness of isolated muscles to temperature and caffeine, (b) sarcoplasmic reticulum (SR) Ca2+ release in single fibers, and (c) oxidative stress and the expression levels of main enzymes involved in the regulation of the redox balance in muscle.
Premarin treatment reduced the temperature and caffeine sensitivity of EDL muscles, normalized SR Ca2+ release, and reduced oxidative stress in males, suggesting that female sex hormones may protect mice from lethal hyperthermic episodes by reducing both the SR Ca2+ leak and oxidative stress.
American Psychological Association (APA)
Michelucci, Antonio& Boncompagni, Simona& Canato, M.& Reggiani, Carlo& Protasi, Feliciano. 2017. Estrogens Protect Calsequestrin-1 Knockout Mice from Lethal Hyperthermic Episodes by Reducing Oxidative Stress in Muscle. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1195369
Modern Language Association (MLA)
Michelucci, Antonio…[et al.]. Estrogens Protect Calsequestrin-1 Knockout Mice from Lethal Hyperthermic Episodes by Reducing Oxidative Stress in Muscle. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1195369
American Medical Association (AMA)
Michelucci, Antonio& Boncompagni, Simona& Canato, M.& Reggiani, Carlo& Protasi, Feliciano. Estrogens Protect Calsequestrin-1 Knockout Mice from Lethal Hyperthermic Episodes by Reducing Oxidative Stress in Muscle. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1195369
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195369