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Cellular Preoxygenation Partially Attenuates the Antitumoral Effect of Cisplatin despite Highly Protective Effects on Renal Epithelial Cells
Joint Authors
Rasoulian, Bahram
Kaeidi, Ayat
Rezaei, Maryam
Hajializadeh, Zahra
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-19
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Our previous in vitro studies demonstrated that oxygen pretreatment significantly protects human embryonic renal tubular cell against acute cisplatin- (CP-) induced cytotoxicity.
The present study was designed to investigate whether this protective effect is associated with decreasing therapeutic effects of cisplatin on malignant cells.
For this purpose, cultured human embryonic kidney epithelial-like (AD293), cervical carcinoma epithelial-like (Hela), and ovarian adenocarcinoma epithelial-like (OVCAR-3) cells were subjected to either 2-hour pretreatment with oxygen (≥90%) or normal air and then to a previously determined 50% lethal dose of cisplatin for 24 hours.
Cellular viability was evaluated via MTT and Neutral Red assays.
Also, activated caspase-3 and Bax/Bcl-2 ratio, as the biochemical markers of cell apoptosis, were determined using immunoblotting.
The hyperoxic preexposure protocol significantly protects renal AD293 cells against cisplatin-induced toxicity.
Oxygen pretreatment also partially attenuated the cisplatin-induced cytotoxic effects on Hela and OVCAR-3 cells.
However, it did not completely protect these cells against the therapeutic cytotoxic effects of cisplatin.
In summary, the protective methods for reducing cisplatin nephrotoxic side effects like oxygen pretreatment might be associated with concurrent reduction of the therapeutic cytotoxic effects of cisplatin on malignant cells like cervical carcinoma (Hela) and ovarian adenocarcinoma (OVCAR-3) cells.
American Psychological Association (APA)
Rasoulian, Bahram& Kaeidi, Ayat& Rezaei, Maryam& Hajializadeh, Zahra. 2017. Cellular Preoxygenation Partially Attenuates the Antitumoral Effect of Cisplatin despite Highly Protective Effects on Renal Epithelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195553
Modern Language Association (MLA)
Rasoulian, Bahram…[et al.]. Cellular Preoxygenation Partially Attenuates the Antitumoral Effect of Cisplatin despite Highly Protective Effects on Renal Epithelial Cells. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1195553
American Medical Association (AMA)
Rasoulian, Bahram& Kaeidi, Ayat& Rezaei, Maryam& Hajializadeh, Zahra. Cellular Preoxygenation Partially Attenuates the Antitumoral Effect of Cisplatin despite Highly Protective Effects on Renal Epithelial Cells. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195553
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195553