REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit
Joint Authors
Fu, Junjiang
Huang, Wenxiang
Li, Lei
Zuo, Qiuhong
Cheng, Shanshan
Bhatti, Muhammad Zeeshan
Xue, Yanyan
Zhang, Yuanyuan
Zhang, Bianhong
Wu, Lin
Chen, Jiwu
Li, Xiaotao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-16
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Hemoglobin (Hb) is a family of proteins in red blood cells responsible for oxygen transport and vulnerable for oxidative damage.
Hemoglobin δ subunit (HBD), a member of Hb family, is normally expressed by cells of erythroid lineage.
Expression of Hb genes has been previously reported in nonerythroid and hematopoietic stem cells.
Here, we report that Hb and HBD can be degraded via REGγ proteasome in hemopoietic tissues and nonerythroid cells.
For this purpose, bone marrow, liver, and spleen hemopoietic tissues from REGγ+/+ and REGγ−/− mice and stable REGγ knockdown cells were evaluated for the degradation of Hb and HBD via REGγ.
Western blot and immunohistochemical analyses exhibited downregulation of Hb in REGγ wild-type mouse tissues.
This was validated by dynamic analysis following blockade of de novo synthesis of proteins with CHX.
Degradation of HBD only occurred in REGγ WT cells but not in REGγN151Y, a dominant-negative REGγ mutant cell.
Notably, downregulation of HBD was found in HeLa shN cells with stimulation of phenylhydrazine, an oxidation inducer, suggesting that the REGγ proteasome may target oxidatively damaged Hbs.
In conclusion, our findings provide important implications for the degradation of Hb and HBD in hemopoietic tissues and nonerythroid cells via the REGγ proteasome.
American Psychological Association (APA)
Zuo, Qiuhong& Cheng, Shanshan& Huang, Wenxiang& Bhatti, Muhammad Zeeshan& Xue, Yanyan& Zhang, Yuanyuan…[et al.]. 2017. REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195575
Modern Language Association (MLA)
Zuo, Qiuhong…[et al.]. REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1195575
American Medical Association (AMA)
Zuo, Qiuhong& Cheng, Shanshan& Huang, Wenxiang& Bhatti, Muhammad Zeeshan& Xue, Yanyan& Zhang, Yuanyuan…[et al.]. REGγ Contributes to Regulation of Hemoglobin and Hemoglobin δ Subunit. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195575
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195575