Mutation Spectrum Induced by 8-Bromoguanine, a Base Damaged by Reactive Brominating Species, in Human Cells
Joint Authors
Sugimura, Haruhiko
Shinmura, Kazuya
Kato, Hisami
Inoue, Yusuke
Nakamura, Satoki
Tao, Hong
Goto, Masanori
Yoshida, Haruki
Tsuzaki, Emi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
To date, the types of mutations caused by 8-bromoguanine (8BrG), a major base lesion induced by reactive brominating species during inflammation, in human cells and the 8BrG repair system remain largely unknown.
In this study, we performed a supF forward mutation assay using a shuttle vector plasmid containing a single 8BrG in three kinds of human cell lines and revealed that 8BrG in DNA predominantly induces a G → T mutation but can also induce G → C, G → A, and delG mutations in human cells.
Next, we tested whether eight kinds of DNA glycosylases (MUTYH, MPG, NEIL1, OGG1, SMUG1, TDG, UNG2, and NTHL1) are capable of repairing 8BrG mispairs with any of the four bases using a DNA cleavage activity assay.
We found that both the SMUG1 protein and the TDG protein exhibit DNA glycosylase activity against thymine mispaired with 8BrG and that the MUTYH protein exhibits DNA glycosylase activity against adenine mispaired with 8BrG.
These results suggest that 8BrG induces some types of mutations, chiefly a G → T mutation, in human cells, and some DNA glycosylases are involved in the repair of 8BrG.
American Psychological Association (APA)
Shinmura, Kazuya& Kato, Hisami& Goto, Masanori& Tao, Hong& Inoue, Yusuke& Nakamura, Satoki…[et al.]. 2017. Mutation Spectrum Induced by 8-Bromoguanine, a Base Damaged by Reactive Brominating Species, in Human Cells. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195583
Modern Language Association (MLA)
Shinmura, Kazuya…[et al.]. Mutation Spectrum Induced by 8-Bromoguanine, a Base Damaged by Reactive Brominating Species, in Human Cells. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1195583
American Medical Association (AMA)
Shinmura, Kazuya& Kato, Hisami& Goto, Masanori& Tao, Hong& Inoue, Yusuke& Nakamura, Satoki…[et al.]. Mutation Spectrum Induced by 8-Bromoguanine, a Base Damaged by Reactive Brominating Species, in Human Cells. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1195583
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195583