![](/images/graphics-bg.png)
Inhibition of miR-302 Suppresses Hypoxia-Reoxygenation-Induced H9c2 Cardiomyocyte Death by Regulating Mcl-1 Expression
Joint Authors
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
MicroRNAs play important roles in cell proliferation, differentiation, and apoptosis, and their expression influences cardiomyocyte apoptosis resulting from ischemia-induced myocardial infarction.
Here, we determined the role of miR expression in cardiomyocyte apoptosis during hypoxia and reoxygenation.
The rat cardiomyocyte cell line H9c2 was incubated for 3 h in normal or hypoxia medium, followed by reoxygenation for 24 h and transfection with a miR-302 mimic or antagomir.
The effect of miR-302 on myeloid leukemia cell-differentiation protein-1 (Mcl-1) expression was determined by western blot, real-time polymerase chain reaction, and luciferase reporter assays, with cell viability assays.
We observed that miR-302 expression was elevated by hypoxia/reoxygenation injury and increased further or decreased by transfection of the miR-302 mimic or miR-302 antagomir, respectively.
Additionally, elevated miR-302 levels increased apoptosis-related protein levels and cardiomyocyte apoptosis, and luciferase reporter assays revealed miR-302 binding to the Mcl-1 mRNA 3′ untranslated region.
Our findings suggested that miR-302 overexpression aggravated hypoxia/reoxygenation-mediated cardiomyocyte apoptosis by inhibiting antiapoptotic Mcl-1 expression, thereby activating proapoptotic molecules.
Furthermore, results indicating cardiomyocyte rescue from hypoxia/reoxygenation injury following treatment with miR-302 antagomir suggested that miR-302 inhibition might constitute a therapeutic strategy for protection against cardiomyocyte apoptosis during hypoxia/reoxygenation injury.
American Psychological Association (APA)
Fang, Yao-Ching& Yeh, Chi-Hsiao. 2017. Inhibition of miR-302 Suppresses Hypoxia-Reoxygenation-Induced H9c2 Cardiomyocyte Death by Regulating Mcl-1 Expression. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1195849
Modern Language Association (MLA)
Fang, Yao-Ching& Yeh, Chi-Hsiao. Inhibition of miR-302 Suppresses Hypoxia-Reoxygenation-Induced H9c2 Cardiomyocyte Death by Regulating Mcl-1 Expression. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1195849
American Medical Association (AMA)
Fang, Yao-Ching& Yeh, Chi-Hsiao. Inhibition of miR-302 Suppresses Hypoxia-Reoxygenation-Induced H9c2 Cardiomyocyte Death by Regulating Mcl-1 Expression. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1195849
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1195849