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Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis
Joint Authors
Gao, Yuan
Wake, Hidenori
Morioka, Yuta
Liu, Keyue
Teshigawara, Kiyoshi
Shibuya, Megumi
Zhou, Jingxiu
Mori, Shuji
Takahashi, Hideo
Nishibori, Masahiro
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Advanced glycation end products (AGEs) are the products of a series of nonenzymatic modifications of proteins by reducing sugars.
AGEs play a pivotal role in development of diabetic complications and atherosclerosis.
Accumulation of AGEs in a vessel wall may contribute to the development of vascular lesions.
Although AGEs have a diverse range of bioactivities, the clearance process of AGEs from the extracellular space, including the incorporation of AGEs into specific cells, subcellular localization, and the fate of AGEs, remains unclear.
In the present study, we examined the kinetics of the uptake of AGEs by mouse macrophage J774.1 cells in vitro and characterized the process.
We demonstrated that AGEs bound to the surface of the cells and were also incorporated into the cytoplasm.
The temperature- and time-dependent uptake of AGEs was saturable with AGE concentration and was inhibited by cytochalasin D but not chlorpromazine.
We also observed the granule-like appearance of AGE immunoreactivity in subcellular localizations in macrophages.
Higher concentrations of AGEs induced intracellular ROS and 4-HNE, which were associated with activation of the NF-κB pathway and caspase-3.
These results suggest that incorporation of AGEs occurred actively by endocytosis in macrophages, leading to apoptosis of these cells through NF-κB activation.
American Psychological Association (APA)
Gao, Yuan& Wake, Hidenori& Morioka, Yuta& Liu, Keyue& Teshigawara, Kiyoshi& Shibuya, Megumi…[et al.]. 2017. Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1196090
Modern Language Association (MLA)
Gao, Yuan…[et al.]. Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1196090
American Medical Association (AMA)
Gao, Yuan& Wake, Hidenori& Morioka, Yuta& Liu, Keyue& Teshigawara, Kiyoshi& Shibuya, Megumi…[et al.]. Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1196090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196090