Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells
Joint Authors
Li, Chia-Jung
Pang, Cheng-Yoong
Chen, Po-Kong
Sun, Li-Yi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-17
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Excessive reactive oxygen species is the major component of a harsh microenvironment after ischemia/reperfusion injury in human tissues.
Combined treatment of N-acetyl-L-cysteine (NAC) and L-ascorbic acid 2-phosphate (AAP) promoted the growth of human mesenchymal stem cells (hMSCs) and suppressed oxidative stress-induced cell death by enhancing mitochondrial integrity and function in vitro.
In this study, we aimed to determine whether NAC and AAP (termed MCA) could enhance the therapeutic potential of hMSCs.
We established a coculture system consisting of MCA-treated and H2O2-treated hMSCs and investigated the role of tunneling nanotubes (TNTs) in the exchange of mitochondria between the 2 cell populations.
The consequences of mitochondria exchange were assessed by fluorescence confocal microscopy and flow cytometry.
The results showed that MCA could increase the mitochondrial mass, respiratory capacity, and numbers of TNTs in hMSCs.
The “energized” mitochondria were transferred to the injured hMSCs via TNTs, the oxidative stress was decreased, and the mitochondrial membrane potential of the H2O2-treated hMSCs was stabilized.
The transfer of mitochondria decreased the expression of S616-phosphorylated dynamin-related protein 1, a protein that dictates the fragmentation/fission of mitochondria.
Concurrently, MCA also enhanced mitophagy in the coculture system, implicating that damaged mitochondria were eliminated in order to maintain cell physiology.
American Psychological Association (APA)
Li, Chia-Jung& Chen, Po-Kong& Sun, Li-Yi& Pang, Cheng-Yoong. 2017. Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1196141
Modern Language Association (MLA)
Li, Chia-Jung…[et al.]. Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1196141
American Medical Association (AMA)
Li, Chia-Jung& Chen, Po-Kong& Sun, Li-Yi& Pang, Cheng-Yoong. Enhancement of Mitochondrial Transfer by Antioxidants in Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1196141
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196141