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Selaginella bryopteris Aqueous Extract Improves Stability and Function of Cryopreserved Human Mesenchymal Stem Cells
Joint Authors
Rizvanov, Albert A.
Bissoyi, Akalabya
Kumar, Awanish
Patra, Pradeep Kumar
Bit, Arindam
Kiassov, Andrey P.
Singh, Abhishek Kumar
Jha, Anubhuti
Ojha, Archit
Bhoi, Pooja
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The effective long-term cryopreservation of human mesenchymal stem cells (MSCs) is an essential prerequisite step and represents a critical approach for their sustained supply in basic research, regenerative medicine, and tissue engineering applications.
Therefore, attempts have been made in the present investigation to formulate a freezing solution consisting of a combination of Selaginella bryopteris water-soluble extract with and without dimethyl sulfoxide (Me2SO) for the efficient long-term storage of human umbilical cord blood- (hUCB-) derived MSCs.
The cryopreservation experiment using the formulated freezing solution was further performed with hUCB MSCs in a controlled rate freezer.
A significant increase in postthaw cell viability and cell attachment of MSCs was achieved with freezing medium containing Selaginella bryopteris water extract along with 10% Me2SO as compared to the freezing medium containing Me2SO (10% v/v) alone.
Furthermore, the decreasing apoptotic events and reactive oxygen species production along with increasing expression of heat shock proteins also confirmed the beneficial effect of Selaginella bryopteris water extract.
The beneficial effect of Selaginella bryopteris water extract was validated by its ability to render postpreservation high cell viability.
In conclusion, the formulated freezing solution has been demonstrated to be effective for the standardization of cryopreservation protocol for hMSCs.
American Psychological Association (APA)
Singh, Abhishek Kumar& Jha, Anubhuti& Bit, Arindam& Kiassov, Andrey P.& Rizvanov, Albert A.& Ojha, Archit…[et al.]. 2017. Selaginella bryopteris Aqueous Extract Improves Stability and Function of Cryopreserved Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1196199
Modern Language Association (MLA)
Singh, Abhishek Kumar…[et al.]. Selaginella bryopteris Aqueous Extract Improves Stability and Function of Cryopreserved Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1196199
American Medical Association (AMA)
Singh, Abhishek Kumar& Jha, Anubhuti& Bit, Arindam& Kiassov, Andrey P.& Rizvanov, Albert A.& Ojha, Archit…[et al.]. Selaginella bryopteris Aqueous Extract Improves Stability and Function of Cryopreserved Human Mesenchymal Stem Cells. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1196199
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196199