Hydrogen Sulfide Inhibits Autophagic Neuronal Cell Death by Reducing Oxidative Stress in Spinal Cord Ischemia Reperfusion Injury
Joint Authors
Li, Changwei
Xie, Lei
Yu, Sifei
Liang, Yu
Yang, Kai
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-08
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Autophagy is upregulated in spinal cord ischemia reperfusion (SCIR) injury; however, its expression mechanism is largely unknown; moreover, whether autophagy plays a neuroprotective or neurodegenerative role in SCIR injury remains controversial.
To explore these issues, we created an SCIR injury rat model via aortic arch occlusion.
Compared with normal controls, autophagic cell death was upregulated in neurons after SCIR injury.
We found that autophagy promoted neuronal cell death during SCIR, shown by a significant number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling- (TUNEL-) positive cells colabeled with the autophagy marker microtubule-associated protein 1 light chain 3, while the autophagy inhibitor 3-methyladenine reduced the number of TUNEL-positive cells and restored neurological and motor function.
Additionally, we showed that oxidative stress was the main trigger of autophagic neuronal cell death after SCIR injury and N-acetylcysteine inhibited autophagic cell death and restored neurological and motor function in SCIR injury.
Finally, we found that hydrogen sulfide (H2S) inhibited autophagic cell death significantly by reducing oxidative stress in SCIR injury via the AKT-the mammalian target of rapamycin (mTOR) pathway.
These findings reveal that oxidative stress induces autophagic cell death and that H2S plays a neuroprotective role by reducing oxidative stress in SCIR.
American Psychological Association (APA)
Xie, Lei& Yu, Sifei& Yang, Kai& Li, Changwei& Liang, Yu. 2017. Hydrogen Sulfide Inhibits Autophagic Neuronal Cell Death by Reducing Oxidative Stress in Spinal Cord Ischemia Reperfusion Injury. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1196235
Modern Language Association (MLA)
Xie, Lei…[et al.]. Hydrogen Sulfide Inhibits Autophagic Neuronal Cell Death by Reducing Oxidative Stress in Spinal Cord Ischemia Reperfusion Injury. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-15.
https://search.emarefa.net/detail/BIM-1196235
American Medical Association (AMA)
Xie, Lei& Yu, Sifei& Yang, Kai& Li, Changwei& Liang, Yu. Hydrogen Sulfide Inhibits Autophagic Neuronal Cell Death by Reducing Oxidative Stress in Spinal Cord Ischemia Reperfusion Injury. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-15.
https://search.emarefa.net/detail/BIM-1196235
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196235