Green Tea Polyphenols Ameliorate the Early Renal Damage Induced by a High-Fat Diet via KetogenesisSIRT3 Pathway
Joint Authors
Yi, Weijie
Xie, Xiao
Du, Miying
Bu, Yongjun
Wu, Nannan
Yang, Hui
Tian, Chong
Xu, Fangyi
Xiang, Siyun
Zhang, Piwei
Chen, Zhuo
Zuo, Xuezhi
Ying, Chenjiang
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-26
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Scope.
Several reports in the literature have suggested the renoprotective effects of ketone bodies and green tea polyphenols (GTPs).
Our previous study found that GTP consumption could elevate the renal expression of the ketogenic rate-limiting enzyme, which was decreased by a high-fat diet (HFD) in rats.
Here, we investigated whether ketogenesis can mediate renoprotection by GTPs against an HFD.
Methods and Results.
Wistar rats were fed a standard or HFD with or without GTPs for 18 weeks.
The renal oxidative stress level, kidney function, renal expression, and activity levels of mitochondrial 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase 2 (HMGCS2) and sirtuin 3(SIRT3) were detected.
The increased renal oxidative stress and the loss of renal function induced by the HFD were ameliorated by GTPs.
Renal ketogenesis and SIRT3 expression and activity levels, which were reduced by the HFD, were restored by GTPs.
In vitro, HEK293 cells were transfected with the eukaryotic expression plasmid pcDNA HMGCS2.
GTP treatment could upregulate HMGCS2 and SIRT3 expression.
Although SIRT3 expression was not affected by HMGCS2 transfection, the 4-hydroxy-2-nonenal (4-HNE) level and the acetyl-MnSOD (K122)/MnSOD ratio were reduced in HMGCS2-transfected cells in the context of H2O2.
Conclusion.
The ketogenesis/SIRT3 pathway mediates the renoprotection of GTPs against the oxidative stress induced by an HFD.
American Psychological Association (APA)
Yi, Weijie& Xie, Xiao& Du, Miying& Bu, Yongjun& Wu, Nannan& Yang, Hui…[et al.]. 2017. Green Tea Polyphenols Ameliorate the Early Renal Damage Induced by a High-Fat Diet via KetogenesisSIRT3 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1196332
Modern Language Association (MLA)
Yi, Weijie…[et al.]. Green Tea Polyphenols Ameliorate the Early Renal Damage Induced by a High-Fat Diet via KetogenesisSIRT3 Pathway. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1196332
American Medical Association (AMA)
Yi, Weijie& Xie, Xiao& Du, Miying& Bu, Yongjun& Wu, Nannan& Yang, Hui…[et al.]. Green Tea Polyphenols Ameliorate the Early Renal Damage Induced by a High-Fat Diet via KetogenesisSIRT3 Pathway. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1196332
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196332