Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker
Joint Authors
Porro, Benedetta
Squellerio, Isabella
Werba, José Pablo
Tremoli, Elena
Cavalca, Viviana
Turnu, Linda
Di Minno, Alessandro
Bonomi, Alice
Manega, Chiara Maria
Parolari, Alessandro
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Coronary artery bypass grafting (CABG), one of the most common cardiac surgical procedures, is characterized by a burst of oxidative stress.
8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), produced following DNA repairing, is used as an indicator of oxidative DNA damage in humans.
The effect of CABG on oxidative-induced DNA damage, evaluated through the measurement of urinary 8-oxodG by a developed and validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method in 52 coronary artery disease (CAD) patients, was assessed before (T0), five days (T1), and six months (T2) after CABG procedure.
These results were compared with those obtained in 40 subjects with cardiovascular risk factors and without overt cardiovascular disease (CTR).
Baseline (T0) 8-oxodG was higher in CAD than in CTR (p=0.035).
A significant burst was detected at T1 (p=0.019), while at T2, 8-oxodG levels were significantly lower than those measured at T0 (p<0.0001) and comparable to those found in CTR (p=0.73).
A similar trend was observed for urinary 8-iso-prostaglandin F2α (8-isoPGF2α), a reliable marker of oxidative stress.
In the whole population baseline, 8-oxodG significantly correlated with 8-isoPGF2α levels (r=0.323, p=0.002).
These data argue for CABG procedure in CAD patients as inducing a short-term increase in oxidative DNA damage, as revealed by 8-oxodG concentrations, and a long-term return of such metabolite toward physiological levels.
American Psychological Association (APA)
Turnu, Linda& Di Minno, Alessandro& Porro, Benedetta& Squellerio, Isabella& Bonomi, Alice& Manega, Chiara Maria…[et al.]. 2017. Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker. Oxidative Medicine and Cellular Longevity،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1196670
Modern Language Association (MLA)
Turnu, Linda…[et al.]. Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker. Oxidative Medicine and Cellular Longevity No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1196670
American Medical Association (AMA)
Turnu, Linda& Di Minno, Alessandro& Porro, Benedetta& Squellerio, Isabella& Bonomi, Alice& Manega, Chiara Maria…[et al.]. Assessing Free-Radical-Mediated DNA Damage during Cardiac Surgery: 8-Oxo-7,8-dihydro-2′-deoxyguanosine as a Putative Biomarker. Oxidative Medicine and Cellular Longevity. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1196670
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196670