ATF4 Involvement in TLR4 and LOX-1-Induced Host Inflammatory Response to Aspergillus fumigatus Keratitis
Joint Authors
Zhang, Shuchao
Meng, Pingping
Liu, Guibo
Liu, Kuixiang
Che, Chengye
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-13
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
Activating transcription factor 4 (ATF4) is induced by various stressors.
Here, we investigated the expression of ATF4 in the host inflammatory response to Aspergillus fumigatus (A.
fumigatus) keratitis.
Methods.
A.
fumigatus keratitis mouse models developed by intrastromal injection as well as corneal epithelium scratching were examined daily with a slit lamp microscope for corneal opacification and ulceration.
Subsequent in vitro experimentation was carried out in human corneal epithelial cells (HCECs) as well as THP-1 macrophages infected with A.
fumigatus.
Inhibitors, including CLI-095, Poly (I), SCH772984, and SP600125, were used to assess the role of proteins like toll-like receptor 4 (TLR4), lectin-type oxidized LDL receptor 1 (LOX-1), extracellular signal-regulated kinases (ERK1/2), and c-Jun N-terminal kinase (JNK) in ATF4 expression as a response to A.
fumigatus infection.
This assessment was made in both mouse models and HCECs using western blot.
Results.
Compared to the controls, ATF4 was increased in corneas from two kinds of A.
fumigatus keratitis models at 3 days after infection.
ATF4 expression was upregulated with A.
fumigatus conidia both in HCECs and THP-1 macrophages 16 hours after stimulation.
Furthermore, ATF4 expression in response to A.
fumigatus infection was shown to be dependent on TLR4 and LOX-1 expression, and ERK1/2 and JNK contributed to the expression of ATF4 in response to A.
fumigatus.
Conclusion.
Our results clearly indicate that ATF4 was involved in the host antifungal immune response to A.
fumigatus keratitis; expression was found to be dependent on TLR4, LOX-1 expression, and MAPKs pathway.
American Psychological Association (APA)
Zhang, Shuchao& Meng, Pingping& Liu, Guibo& Liu, Kuixiang& Che, Chengye. 2018. ATF4 Involvement in TLR4 and LOX-1-Induced Host Inflammatory Response to Aspergillus fumigatus Keratitis. Journal of Ophthalmology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1196704
Modern Language Association (MLA)
Zhang, Shuchao…[et al.]. ATF4 Involvement in TLR4 and LOX-1-Induced Host Inflammatory Response to Aspergillus fumigatus Keratitis. Journal of Ophthalmology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1196704
American Medical Association (AMA)
Zhang, Shuchao& Meng, Pingping& Liu, Guibo& Liu, Kuixiang& Che, Chengye. ATF4 Involvement in TLR4 and LOX-1-Induced Host Inflammatory Response to Aspergillus fumigatus Keratitis. Journal of Ophthalmology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1196704
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196704