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Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy
Joint Authors
Rodrigues, Eduardo Buchele
Maia, Mauricio
Peris, Cristiane S.
Caiado, Rafael R.
Souza Lima-Filho, Acácio Alves
Gonçalves, Mariana Batista
de Queiroz Alves, Bruno
Palma Urushima, Joao Guilherme
Ragazzi, Raul
Farah, Michel Eid
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Purpose.
To classify and quantify anthocyanins in a vital dye extracted from the acai fruit (Euterpe oleracea), adjust pH and osmolarity, and perform lyophilization to develop a new chromovitrectomy dye.
Methods.
Three dye concentrations 10%, 25%, and 35% (equivalent to 100, 250, and 350 mg of lyophilized acai fruit pulp extract samples) were evaluated when diluted in 1 ml of phosphate-buffered solution (pH 7 and 300 mOsm).
The dye was analyzed by mass spectrometry and high-performance liquid chromatography (HPLC) to identify and quantify anthocyanins molecules.
Results.
The pH and osmolarity correction and lyophilization were performed without damaging the anthocyanin molecular structure.
Mass spectrometry confirmed the presence of five anthocyanins in the three concentrations of the dye.
Cyanidin-3-O-glucoside was the major anthocyanin found.
HPLC showed that the concentration of anthocyanin was similar, independent of the dye concentration tested.
Conclusions.
Lyophilization and the correction of pH and osmolarity (7.00 and 300 mOsm, resp.) were performed successfully.
Five anthocyanins are present in the dye from the acai fruit.
The major anthocyanin is cyanidin-3-O-glucoside.
Independent of the dye concentration tested, the anthocyanin concentration was similar.
Standardized chemical characteristics of this new dye may allow its use during chromovitrectomy in humans.
American Psychological Association (APA)
Peris, Cristiane S.& Caiado, Rafael R.& Souza Lima-Filho, Acácio Alves& Rodrigues, Eduardo Buchele& Farah, Michel Eid& Gonçalves, Mariana Batista…[et al.]. 2018. Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy. Journal of Ophthalmology،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1196802
Modern Language Association (MLA)
Peris, Cristiane S.…[et al.]. Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy. Journal of Ophthalmology No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1196802
American Medical Association (AMA)
Peris, Cristiane S.& Caiado, Rafael R.& Souza Lima-Filho, Acácio Alves& Rodrigues, Eduardo Buchele& Farah, Michel Eid& Gonçalves, Mariana Batista…[et al.]. Analysis of Anthocyanins Extracted from the Acai Fruit (Euterpe oleracea): A Potential Novel Vital Dye for Chromovitrectomy. Journal of Ophthalmology. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1196802
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1196802