DNA Methylation Status of the Interspersed Repetitive Sequences for LINE-1, Alu, HERV-E, and HERV-K in Trabeculectomy Specimens from Glaucoma Eyes
Joint Authors
Prombhul, Sasiprapa
Chansangpetch, Sunee
Manassakorn, Anita
Tantisevi, Visanee
Sodsai, Pimpayao
Hirankarn, Nattiya
Lin, Shan C.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background/Aims.
Epigenetic mechanisms via DNA methylation may be related to glaucoma pathogenesis.
This study aimed to determine the global DNA methylation level of the trabeculectomy specimens among patients with different types of glaucoma and normal subjects.
Methods.
Trabeculectomy sections from 16 primary open-angle glaucoma (POAG), 12 primary angle-closure glaucoma (PACG), 16 secondary glaucoma patients, and 10 normal controls were assessed for DNA methylation using combined-bisulfite restriction analysis.
The percentage of global methylation level of the interspersed repetitive sequences for LINE-1, Alu, HERV-E, and HERV-K were compared between the 4 groups.
Results.
There were no significant differences in the methylation for LINE-1 and HERV-E between patients and normal controls.
For the Alu marker, the methylation was significantly lower in all types of glaucoma patients compared to controls (POAG 52.19% versus control 52.83%, p=0.021; PACG 51.50% versus control, p=0.005; secondary glaucoma 51.95% versus control, p=0.014), whereas the methylation level of HERV-K was statistically higher in POAG patients compared to controls (POAG 49.22% versus control 48.09%, p=0.017).
Conclusions.
The trabeculectomy sections had relative DNA hypomethylation of Alu in all glaucoma subtypes and relative DNA hypermethylation of HERV-K in POAG patients.
These methylation changes may lead to the fibrotic phenotype in the trabecular meshwork.
American Psychological Association (APA)
Chansangpetch, Sunee& Prombhul, Sasiprapa& Tantisevi, Visanee& Sodsai, Pimpayao& Manassakorn, Anita& Hirankarn, Nattiya…[et al.]. 2018. DNA Methylation Status of the Interspersed Repetitive Sequences for LINE-1, Alu, HERV-E, and HERV-K in Trabeculectomy Specimens from Glaucoma Eyes. Journal of Ophthalmology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1197098
Modern Language Association (MLA)
Chansangpetch, Sunee…[et al.]. DNA Methylation Status of the Interspersed Repetitive Sequences for LINE-1, Alu, HERV-E, and HERV-K in Trabeculectomy Specimens from Glaucoma Eyes. Journal of Ophthalmology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1197098
American Medical Association (AMA)
Chansangpetch, Sunee& Prombhul, Sasiprapa& Tantisevi, Visanee& Sodsai, Pimpayao& Manassakorn, Anita& Hirankarn, Nattiya…[et al.]. DNA Methylation Status of the Interspersed Repetitive Sequences for LINE-1, Alu, HERV-E, and HERV-K in Trabeculectomy Specimens from Glaucoma Eyes. Journal of Ophthalmology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1197098
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197098