Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder
Joint Authors
Vallderiola, Francesc
Tolosa, Eduardo S.
Morén, C.
González-Casacuberta, Í.
Navarro-Otano, J.
Juárez-Flores, D.
Vilas, D.
Garrabou, G.
Milisenda, J. C.
Pont-Sunyer, C.
Catalán-García, M.
Guitart-Mampel, M.
Tobías, E.
Cardellach, F.
Iranzo, A.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
To determine potential mitochondrial and oxidative alterations in colon biopsies from idiopathic REM sleep behavior disorder (iRBD) and Parkinson’s disease (PD) subjects.
Methods.
Colonic biopsies from 7 iRBD subjects, 9 subjects with clinically diagnosed PD, and 9 healthy controls were homogenized in 5% w/v mannitol.
Citrate synthase (CS) and complex I (CI) were analyzed spectrophotometrically.
Oxidative damage was assessed either by lipid peroxidation, through malondialdehyde and hydroxyalkenal content by spectrophotometry, or through antioxidant enzyme levels of superoxide dismutase-2 (SOD2), glutathione peroxidase-1 (Gpx1), and catalase (CAT) by western blot.
The presence of mitochondrial DNA (mtDNA) deletions was assessed by long PCR and electrophoresis.
Results.
Nonsignificant trends to CI decrease in both iRBD (45.69±18.15; 23% decrease) and PD patients (37.57±12.41; 37% decrease) were found compared to controls (59.51±12.52, p: NS).
Lipid peroxidation was maintained among groups (iRBD: 27.46±3.04, PD: 37.2±3.92, and controls: 31.71±3.94; p: NS).
Antioxidant enzymes SOD2 (iRBD: 2.30±0.92, PD: 1.48±0.39, and controls: 1.09±0.318) and Gpx1 (iRBD 0.29±0.12, PD: 0.56±0.33, and controls: 0.38±0.16) did not show significant differences between groups.
CAT was only detected in 2 controls and 1 iRBD subject.
One iRBD patient presented a single mtDNA deletion.
American Psychological Association (APA)
Morén, C.& González-Casacuberta, Í.& Navarro-Otano, J.& Juárez-Flores, D.& Vilas, D.& Garrabou, G.…[et al.]. 2017. Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder. Parkinson’s Disease،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197167
Modern Language Association (MLA)
Morén, C.…[et al.]. Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder. Parkinson’s Disease No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1197167
American Medical Association (AMA)
Morén, C.& González-Casacuberta, Í.& Navarro-Otano, J.& Juárez-Flores, D.& Vilas, D.& Garrabou, G.…[et al.]. Colonic Oxidative and Mitochondrial Function in Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder. Parkinson’s Disease. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1197167
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197167