Association of PPAR Alpha Intron 7 GC, PPAR Gamma 2 Pro12Ala, and C161T Polymorphisms with Serum Fetuin-A Concentrations

Abstract EN

Background.

Both peroxisome activator proteins (PPARs) and fetuin-A play a role in lipid and glucose metabolism.

Aims.

We investigated whether PPARα intron 7 G2468/C and PPARγ2 Pro12Ala and PPARγ exon 6 C161T polymorphisms are associated with serum fetuin-A concentrations.

Patients and Methods.

The PPARα intron 7 G/C polymorphism was studied in cohort 1 (79 reference individuals, 165 postinfarction patients).

The two PPARγ polymorphisms were investigated in cohort 2 (162 reference individuals, 165 postinfarction patients).

Fetuin-A levels and PPAR polymorphisms were determined by radial immunodiffusion and polymerase chain reaction-restriction fragment length polymorphism techniques.

Results.

The C allele variant of PPARα intron 7 G2467C was associated with higher fetuin-A levels (p=0.018).

Postinfarction status (p=0.001), PPARα intron 7 GG/GC/CC genotypes (p=0.032), and the C allele (p=0.021) were the strongest determinants of fetuin-A concentration in a multiple regression model.

Higher fetuin-A levels were associated with the Pro variant of PPARγ2 (p=0.047).

Postinfarction status (p=0.041) and BMI (p<0.001) but not PPARγ2 Pro were the strongest determinants of fetuin-A concentrations.

PPARγ exon 6 C161T genotypes were not associated with fetuin-A levels.

Conclusions.

Fetuin-A was determined mainly by the PPARα intron 7C allele and postinfarction status in cohort 1 and the BMI and postinfarction in cohort 2.

The PPARα intron 7C and PPARγ2 Pro variants are associated with fetuin-A levels.