Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy
Joint Authors
Takahashi, Hideki
Yamamoto, Tatsuo
Yamazaki, Motomi
Murase, Takayuki
Matsuno, Takayuki
Chishima, Fumihisa
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Aim.
To investigate the changes in the maternal immune system at term pregnancy, we studied the expression of natural cytotoxicity receptors (NCRs) and the cytokine production of NK cells in term placenta decidua and peripheral blood.
Methods.
Term decidua and peripheral blood were taken from patients undergoing elective cesarean section.
The lymphocytes were separated using density gradient centrifugation (DGC) from peripheral blood and were separated from decidua using DGC after enzyme digestion.
These cells were stained with FITC anti-CD56 and Per-CP anti-CD3 monoclonal antibodies, and the NCRs were stained with PE-conjugated anti-NKG2D, NKp46, NKp30, and NKp44 monoclonal antibodies.
Cytokines, including IFN-γ, TNF-α, IL-10, and TGF-β, were stained and then analyzed by flow cytometry.
Results.
There were fewer cells positive for NKG2D, NKp46, and NKp30 among CD56+CD3- cells in deciduas than in peripheral blood, but the percentages of NKp44-positive cells in CD56+CD3- lymphocytes in deciduas tended to be higher.
Conclusion.
The decreased expression of some NCRs in deciduas may be related to decreased cytotoxicity at term pregnancy, but the increased expression of NKp44 may affect the increased cytokine production in the decidua.
Similarly, the expression of NCRs in the decidua may be connected to the maintenance of pregnancy at term.
American Psychological Association (APA)
Takahashi, Hideki& Yamamoto, Tatsuo& Yamazaki, Motomi& Murase, Takayuki& Matsuno, Takayuki& Chishima, Fumihisa. 2018. Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy. Journal of Pregnancy،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1197356
Modern Language Association (MLA)
Takahashi, Hideki…[et al.]. Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy. Journal of Pregnancy No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1197356
American Medical Association (AMA)
Takahashi, Hideki& Yamamoto, Tatsuo& Yamazaki, Motomi& Murase, Takayuki& Matsuno, Takayuki& Chishima, Fumihisa. Natural Cytotoxicity Receptors in Decidua Natural Killer Cells of Term Normal Pregnancy. Journal of Pregnancy. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1197356
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197356