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Comparing the Yield of Staphylococcus aureus Recovery with Static versus Agitated Broth Incubation
Joint Authors
Burnham, Carey-Ann D.
Muenks, Carol E.
Hogan, Patrick G.
Fritz, Stephanie A.
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-3, 3 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-26
Country of Publication
Egypt
No. of Pages
3
Main Subjects
Abstract EN
Given the lack of standardization of methodologies for microbial recovery from built environments, we sought to compare the yield of Staphylococcus aureus with a broth enrichment method when incubated in agitated versus static conditions.
Five unique strains of S.
aureus at five different concentrations were cultured to compare direct plating, agitated broth enrichment, and static broth enrichment culture methods.
All samples were incubated at 35° in ambient air.
The lowest concentration recovered across three replicates and five strains did not differ between culture methods (Fisher’s exact test, p=0.50); notably, recovery of S.
aureus was equivalent between static and agitated broth incubation.
When broth enrichment was used (both static and agitated), the burden of S.
aureus growth was higher (by semiquantitative assessment of 4-quadrant streaking) compared to the direct plating culture method.
Optimizing strategies for microbial recovery is essential, particularly in areas of lower biomass, given the paucity of research concerning microbial communities of built environments.
The results of this study, in conjunction with other experiments investigating microbiomes of built environments, can help inform protocols for standardizing culturing methods within built environments.
American Psychological Association (APA)
Muenks, Carol E.& Hogan, Patrick G.& Burnham, Carey-Ann D.& Fritz, Stephanie A.. 2018. Comparing the Yield of Staphylococcus aureus Recovery with Static versus Agitated Broth Incubation. Journal of Pathogens،Vol. 2018, no. 2018, pp.1-3.
https://search.emarefa.net/detail/BIM-1197467
Modern Language Association (MLA)
Muenks, Carol E.…[et al.]. Comparing the Yield of Staphylococcus aureus Recovery with Static versus Agitated Broth Incubation. Journal of Pathogens No. 2018 (2018), pp.1-3.
https://search.emarefa.net/detail/BIM-1197467
American Medical Association (AMA)
Muenks, Carol E.& Hogan, Patrick G.& Burnham, Carey-Ann D.& Fritz, Stephanie A.. Comparing the Yield of Staphylococcus aureus Recovery with Static versus Agitated Broth Incubation. Journal of Pathogens. 2018. Vol. 2018, no. 2018, pp.1-3.
https://search.emarefa.net/detail/BIM-1197467
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197467