Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain
Joint Authors
Sun, Yi
Li, Haifeng
Zhang, Dengwen
Sun, Qiang
Tian, Y. K.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
This study aimed to investigate the use of human bone marrow mesenchymal stem cells (hBMSCs) genetically engineered with the human proenkephalin (hPPE) gene to treat bone cancer pain (BCP) in a rat model.
Methods.
Primary cultured hBMSCs were passaged and modified with hPPE, and the cell suspensions (6 × 106) were then intrathecally injected into a rat model of BCP.
Paw mechanical withdrawal threshold (PMWT) was measured before and after BCP.
The effects of hPPE gene transfer on hBMSC bioactivity were analyzed in vitro and in vivo.
Results.
No changes were observed in the surface phenotypes and differentiation of hBMSCs after gene transfer.
The hPPE-hBMSC group showed improved PMWT values on the ipsilateral side of rats with BCP from day 12 postoperatively, and the analgesic effect was reversed by naloxone.
The levels of proinflammatory cytokines such as IL-1β and IL-6 were ameliorated, and leucine-enkephalin (L-EK) secretion was augmented, in the hPPE-engineered hBMSC group.
Conclusion.
The intrathecal administration of BMSCs modified with the hPPE gene can effectively relieve pain caused by bone cancer in rats and might be a potentially therapeutic tool for cancer-related pain in humans.
American Psychological Association (APA)
Sun, Yi& Tian, Y. K.& Li, Haifeng& Zhang, Dengwen& Sun, Qiang. 2017. Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain. Pain Research and Management،Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1197469
Modern Language Association (MLA)
Sun, Yi…[et al.]. Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain. Pain Research and Management No. 2017 (2017), pp.1-11.
https://search.emarefa.net/detail/BIM-1197469
American Medical Association (AMA)
Sun, Yi& Tian, Y. K.& Li, Haifeng& Zhang, Dengwen& Sun, Qiang. Antinociceptive Effect of Intrathecal Injection of Genetically Engineered Human Bone Marrow Stem Cells Expressing the Human Proenkephalin Gene in a Rat Model of Bone Cancer Pain. Pain Research and Management. 2017. Vol. 2017, no. 2017, pp.1-11.
https://search.emarefa.net/detail/BIM-1197469
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197469