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Prevalence of Panton-Valentine Leukocidin Gene among Community Acquired Staphylococcus aureus: A Real-Time PCR Study
Joint Authors
Karmakar, Amit
Dua, Parimal
Ghosh, Chandradipa
Jana, Debarati
Dutta, Kunal
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Panton-Valentine leukocidin (luk-pv) is a cytotoxin that causes leukocyte destruction and tissue necrosis.
The aim of this study was to determine the prevalence of the pv1, mecA, and nuc genes in Staphylococcus aureus isolates obtained from anterior nares and superficial infection sites of skin in a slum population of West Bengal, India.
Expression level of pv1 gene was also analysed.
Twenty-two S.
aureus strains were isolated, and phenotype and genotype specific examinations for S.
aureus isolates were carried out.
Molecular identification was done by PCR using species-specific 16S rRNA primer pairs and finally 22 isolates were found to be positive as S.
aureus.
The antibiotic responsiveness of all these isolates and the minimum inhibitory concentration (MIC) of MRSA isolates were determined using the broth dilution method with vancomycin.
Antibiogram analysis of isolated S.
aureus strains with respect to different antimicrobial agents revealed antibiotic resistance ranging from 27 to 91%.
The results of MIC for vancomycin showed 95% of strains to be VSSA and 5% to be VISA.
68% isolates were resistant to methicillin.
All the isolates were subjected to detection of pv1, mecA, and nuc genes, and 9%, 68%, and 27% were found to harbour pvl, mecA, and nuc genes, respectively.
All the MRSA strains produced high to moderate levels of biofilm.
pvl gene expression was carried out in vitro by Real-Time PCR.
The low ∆Ct value (0.493) was indicative of high expression of pvl in one S.
aureus strain.
Thus, detection of pvl gene in community acquired S.
aureus indicates the emergence of pathogenic S.
aureus in community setup in the studied region.
The existing exploration is extremely imperative and informative for the high level multi-drug resistant S.
aureus infections inclusive of MRSA.
American Psychological Association (APA)
Karmakar, Amit& Jana, Debarati& Dutta, Kunal& Dua, Parimal& Ghosh, Chandradipa. 2018. Prevalence of Panton-Valentine Leukocidin Gene among Community Acquired Staphylococcus aureus: A Real-Time PCR Study. Journal of Pathogens،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1197492
Modern Language Association (MLA)
Karmakar, Amit…[et al.]. Prevalence of Panton-Valentine Leukocidin Gene among Community Acquired Staphylococcus aureus: A Real-Time PCR Study. Journal of Pathogens No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1197492
American Medical Association (AMA)
Karmakar, Amit& Jana, Debarati& Dutta, Kunal& Dua, Parimal& Ghosh, Chandradipa. Prevalence of Panton-Valentine Leukocidin Gene among Community Acquired Staphylococcus aureus: A Real-Time PCR Study. Journal of Pathogens. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1197492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197492