“CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica
Joint Authors
De La Garza-Salinas, Laura H.
Vargas-Villarreal, Javier
Meester, Irene
Sampayo-Reyes, A.
Monsivais-Diaz, O.
Barba-Dávila, B. A.
Hernández-García, Magda E.
González-Salazar, Francisco
Guzman-de la Garza, Francisco Javier
Garza-González, Jesús N.
Source
Journal of Parasitology Research
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Amebiasis remains a major health problem in Mexico.
Therefore, the search for better culture media and low-cost diagnostic and therapeutic tools is fundamental.
We present a new culture medium for Entamoeba histolytica which allows the microbe to preserve its virulence factors and ability to induce hepatic abscesses in animal models.
The novel CLUPS medium is an improved version of the PEHPS medium, previously designed in our laboratory.
The main difference is the substitution of raw beef liver in PEHPS by raw beef lung in the CLUPS medium.
To compare the performance of three-culture media (traditional TYI-S-33, PEHPS, and CLUPS), E.
histolytica trophozoites were cultured in quintuplicate, followed by the evaluation of phospholipase activity and the induction of liver abscesses in golden hamsters.
E.
histolytica trophozoites grew significantly better in CLUPS medium than in TYI-S-33.
Likewise, CLUPS-cultured trophozoites produced significantly more phospholipases than TYI-S-33-cultured trophozoites.
Finally, trophozoites grown in any of the three tested media had similar potential to induce liver abscesses.
American Psychological Association (APA)
González-Salazar, Francisco& Meester, Irene& Guzman-de la Garza, Francisco Javier& De La Garza-Salinas, Laura H.& Sampayo-Reyes, A.& Garza-González, Jesús N.…[et al.]. 2018. “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica. Journal of Parasitology Research،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1197570
Modern Language Association (MLA)
González-Salazar, Francisco…[et al.]. “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica. Journal of Parasitology Research No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1197570
American Medical Association (AMA)
González-Salazar, Francisco& Meester, Irene& Guzman-de la Garza, Francisco Javier& De La Garza-Salinas, Laura H.& Sampayo-Reyes, A.& Garza-González, Jesús N.…[et al.]. “CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica. Journal of Parasitology Research. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1197570
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1197570